基于转录组分析玉米幼苗对盐胁迫的响应  

Transcriptome-based analysis of maize seedlings in response to salt stress

作　　者：李涛[1] 屈小玉 马小英 刘威帆 万猛虎 马风兰 吴娜[1] 刘吉利[2] LI Tao;QU Xiaoyu;MA Xiaoying;LIU Weifan;WAN Menghu;MA Fenglan;WU Na;LIU Jili(College of Agriculture,Ningxia University,Yinchuan 750021,China;College of Ecology and Environment,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,银川750021 [2]宁夏大学生态环境学院,银川750021

出　　处：《中国农业大学学报》2025年第1期38-54,共17页Journal of China Agricultural University

基　　金：国家重点研发计划(2021YFD1900603)。

摘　　要：为分析玉米耐盐基因及相关代谢途径,选用玉米品种‘禾育157’(HY157)和‘先玉335’(XY335),设置0(CK)、120(S1)、240 mmol/L NaCl(S_(2))3种处理的盆栽试验,测定不同浓度NaCl处理下玉米幼苗的生长指标和转录组表达情况。结果表明:与CK相比,‘HY 157’在S1和S2处理下分别产生3305和4314个差异表达基因(DEGs),分别鉴定出1900和2457个上调、1405和1857个下调DEGs;‘XY 335’在S1和S2处理下分别产生729和5037个DEGs。其中,293和2500个上调、436和2537个下调DEGs。通过对DEGs进行GO富集分析,2个玉米品种共同的上调DEGs显著富集在过氧化氢、盐胁迫的应答及碳水化合物代谢过程等GO条目。KEGG富集分析表明,差异基因主要参与淀粉和蔗糖代谢、苯丙烷类生物合成、磷酸戊糖途径、苯并恶唑类生物合成、MAPK通路(植物)以及植物激素信号传导等代谢途径。苯丙烷类生物合成途径中,有3个基因在‘XY 335’中特异性地上调表达,‘HY 157’则有5个基因特异性地下调表达。此外,上调的转录因子主要集中在MYB、C2H2、AP2、NAC、bHLH、bZIP、WRKY等基因家族。综上,120和240 mmol/L NaCl胁迫下,‘HY 157’的DEGs以及显著上调表达的转录因子,主要参与调控渗透调节、离子平衡、抗氧化防御等多种适应性反应,从而增强玉米幼苗对盐胁迫的耐受性。In order to analyze the salt tolerance genes and its related metabolic pathways in maize,two maize varieties,‘Heyu 157’(HY157)and‘Xianyu 335’(XY 335),were selected in pot experiment.The experiment was conducted to determine the growth indexes and transcriptome expression of maize seedlings under three different NaCl treatments as follows:0(CK),120(S1)and 240 mmol/L NaCl(S_(2)).The results demonstrated that:In comparison to the control(CK),‘HY 157’variety under S_(1) and S_(2) treatments exhibited 3305 and 4314 differentially expressed genes(DEGs),with 1900 and 2457 being up-regulated and 1405 and 1857 being down-regulated,respectively;‘XY 335’variety under S1 and S_(2) treatments produced 729 and 5037 differentially expressed genes(DEGs),with 293 and 2500 upregulated and 436 and 2537 down-regulated,respectively.GO enrichment analysis of the differentially expressed genes(DEGs)revealed that the two maize varieties co-enriched up-regulated genes were significantly enriched in GO entries such as response to hydrogen peroxide,salt stress and carbohydrate metabolism processes.KEGG enrichment analysis showed that the differentially expressed genes were mainly involved in metabolic pathways such as starch and sucrose metabolism,phenylpropanoid biosynthesis,pentose phosphate pathway,benzoxazole biosynthesis,MAPK signaling pathway(plant)and phytohormone signalling.The results of the genome enrichment analysis indicated that the differentially expressed genes were primarily associated with metabolic pathways,including starch and sucrose metabolism,phenylpropanoid biosynthesis,the pentose phosphate pathway,benzoxazole biosynthesis,mitogen-activated protein kinase(MAPK)pathway(plant)and phytohormone signalling.Three genes involved in the phenylpropane biosynthesis pathway exhibited a specific up-regulation and expression in‘XY 335’,while five genes demonstrated a specific down-regulation and expression in‘HY 157’.Furthermore,the up-regulated transcription factors were predominantly observed in gene fa

关 键 词：玉米 盐胁迫 高通量测序 转录组 转录因子 

分 类 号：S513[农业科学—作物学]