超声/近红外双模态响应型量子点纳米探针用于微血栓诊疗的体外实验研究  

In vitro study of ultrasound/near-infrared bimodal responsive quantum dots nanomolecular probes for microthrombosis diagnosis and treatment

作　　者：江智欣 姜楠[1] 王治文 孙梦营 桂斌 田智全[2] 胡波[1] Jiang Zhixin;Jiang Nan;Wang Zhiwen;Sun Mengying;Gui Bin;Tian Zhiquan;Hu Bo(Department of Ultrasound Imaging,Renmin Hospital of Wuhan University,Wuhan 430060,China;College of Chemistry and Molecular Science,Wuhan University,Wuhan 430072,China)

机构地区：[1]武汉大学人民医院超声影像科,武汉430060 [2]武汉大学化学与分子科学学院,武汉430072

出　　处：《中华核医学与分子影像杂志》2025年第1期29-34,共6页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：国家自然科学基金(82102045)。

摘　　要：目的评估超声/近红外双模态复合纳米级探针精氨酸-甘氨酸-天冬氨酸(RGD)/Ag_(2)Te/全氟戊烷(PFP)@介孔二氧化硅纳米粒(MSN)在微血管疾病诊断和治疗中的应用潜力。方法采用超声声振及碳二亚胺法制备负载RGD、PFP及Ag_(2)Te的纳米探针。测定纳米探针表征;验证探针近红外照射下的成像性能、光热响应能力、探针寻靶能力,检测探针的生物安全性;并行超声和近红外照射治疗,评估其溶栓能力;在近红外二区显像下观察小鼠腹壁微血管显影情况,评估纳米探针微血管显影能力。结果纳米探针粒径为(205.3±2.9)nm,电势为(2.05±0.58)mV。RGD偶联率为(82.27±0.36)%,量子点包封率为(80.80±3.26)%;量子点光稳定性良好。随着纳米探针质量浓度的增加,荧光强度增强,升温效果越明显。超声与近红外照射后,有相对更高的溶栓率。RGD/Ag_(2)Te/PFP@MSN成功实现了对小鼠微血管的近红外二区荧光成像。细胞毒性实验与溶血实验显示该纳米探针具有良好的生物安全性。结论RGD/Ag_(2)Te/PFP@MSN纳米探针是一种有潜力的靶向治疗血栓性疾病的策略,结合超声和近红外的双模态疗法,为微血管栓塞的无创可视化诊断和治疗提供了新的可能。Objective To evaluate the application potential of the bimodal ultrasound/near-infrared(NIR)composite nanoscale probe Arg-Gly-Asp(RGD)/Ag_(2)Te/perfluoropentane(PFP)@mesoporous silica nanoparticles(MSN)in the diagnosis and treatment of microvascular diseases.MethodsNanoprobes loaded with RGD,PFP and Ag_(2)Te were prepared by ultrasound sonication and carbodiimide method.The characterization of the nanoprobes was determined.The imaging performance,photothermal response,and target-seeking ability of the nanoprobes under NIR irradiation were verified.The biosafety of the nanoprobes was examined,and the thrombolytic ability of the nanoprobes was evaluated.The mice were observed to visualize microvessels of the abdominal wall under the NIR-Ⅱimaging,and the microvascular visualization ability of the nanoprobes was evaluated.ResultsThe particle size of nanoprobes was(205.3±2.9)nm and the potential was(2.05±0.58)mV.The coupling rate of the RGD was(82.27±0.36)%,the encapsulation rate of the quantum dots was(80.80±3.26)%,and the photostability of the quantum dots was good.The fluorescence intensity was enhanced with the increase of the mass concentration of RGD/Ag_(2)Te/PFP@MSN,and the warming effect was more obvious.After ultrasound and NIR irradiation,the thrombolysis rate was significantly increased.RGD/Ag_(2)Te/PFP@MSN successfully realized NIR-Ⅱfluorescence imaging of mice microvessels.The cytotoxicity assay and hemolysis assay showed that the probe had a good biosafety.ConclusionThe RGD/Ag_(2)Te/PFP@MSN nanoprobe is a potential strategy for targeted therapy of thrombotic diseases,combining dual-modality therapy of ultrasound and NIR to offer new possibilities for non-invasive and visual diagnosis and treatment of microvascular embolism.

关 键 词：血栓形成 纳米粒子 量子点 超声检查 谱学 近红外线 小鼠 裸 大鼠 

分 类 号：R445.1[医药卫生—影像医学与核医学] R543[医药卫生—诊断学]