粪异杆菌对小鼠肠缺血再灌注损伤的作用  

Role of Allobaculum stercoricanis in mice with intestinal ischemia-reperfusion injury

作　　者：王一帆 甘露 徐诺 黄丽萍 罗俊丽 李偲[1] WANG Yi-fan;GAN Lu;XU Nuo;HUANG Li-ping;LUO Jun-li;LI Cai(Department of Anesthesiology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,Guangdong,China;不详)

机构地区：[1]南方医科大学南方医院麻醉科,广东广州510515 [2]成都市第五人民医院麻醉科,四川成都611100

出　　处：《广东医学》2025年第1期21-26,共6页Guangdong Medical Journal

基　　金：国家自然科学基金面上项目(82072224)。

摘　　要：目的观察粪异杆菌处理对小鼠肠缺血再灌注损伤后肠及肠外器官的影响。方法选取SPF级C57/BL6J小鼠18只,随机分为为假手术组(Sham组)、肠缺血再灌注损伤组(I/R组)、粪异杆菌灌胃处理组(A.S+I/R组),每组6只。I/R组和A.S+I/R组建立肠I/R模型。观察小鼠小肠、肝、肺组织形态学变化;用测定小鼠小肠组织及血清中炎症因子白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α(TNF-α)水平,测定小肠组织中肠屏障标记物咬合蛋白(Occludin)、带状闭合蛋白(ZO-1)的水平;评估小鼠肠屏障通透性;测定小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)含量及肺干湿重比。结果粪异杆菌灌胃减轻肠I/R损伤引起的小肠、肝、肺病理学改变,降低小肠病理评分(P<0.001),降低肠组织炎症因子mRNA水平(P<0.05),降低血清中炎症因子释放(P<0.05),改善肠屏障功能(P<0.05),降低ALT、AST含量及肺干湿重比(P<0.05)。结论粪异杆菌处理可以减轻肠I/R后小肠及肠外器官肝、肺损伤,为肠缺血再灌注损伤提供新的预防及治疗思路。Objective To investigate the effects of Allobaculum stercoricanis on intestinal and extra-intestinal organ injuries following intestinal ischemia-reperfusion(I/R)in mice.Methods Eighteen SPF-grade C57/BL6J mice were randomly divided into the sham-operated group(Sham group),intestinal ischemia-reperfusion injury group(I/R group),and Allobaculum stercoricanis gavage treatment group(A.S+I/R group),with six mice in each group.I/R models were established in the I/R group and A.S+I/R group.The study assessed histological changes in the small intestine,liver,and lung tissues;inflammatory markers[interleuhine(IL)-1β,IL-6,tumor necrosis factor-α(TNF-α)]in intestinal tissues and serum;intestinal barrier markers(occludin,ZO-1);gut permeability;serum ALT and AST levels;and lung wet-to-dry weight ratio.Results Allobaculum stercoricanis gavage alleviated pathological changes in the small intestine,liver,and lungs caused by intestinal I/R injury,significantly reducing small intestine pathology scores(P<0.001),inflammatory cytokine mRNA levels in intestinal tissues(P<0.05),and systemic cytokine levels in serum(P<0.05).It improved intestinal barrier integrity(P<0.05),reduced ALT and AST levels,and lowered the lung wet-to-dry weight ratio(P<0.05).Conclusion Allobaculum stercoricanis treatment mitigates intestinal and extra-intestinal organ injuries(liver and lung)following intestinal ischemia-reperfusion injury,offering a new avenue for the prevention and treatment of I/R injuries.

关 键 词：粪异杆菌 肠缺血再灌注损伤 肠道菌群 

分 类 号：R641.27[医药卫生—外科学] R574.5[医药卫生—临床医学]