Allelic variation in the promoter of WRKY22 enhances humid adaptation of Arabidopsis thaliana  

作　　者：Ruyun Liang Luna Tan Xiang Guo Shangling Lou Xuming Dan Yu Han Cheng Zeng Han Zhang Kai Yang Liyang Chen Xin Liang Meng Liu Mengyun Guo Kangqun Yin Si Tang Yan Song Xuemeng Gao Shaobo Gu Jing Hou Yingjun Yao Ruijia Zhang Jin Yan Wensen Fu Xuerui Li Yongqi Hu Yao Liu Wei Liu Qiusai Wu Zhen Yan Weitao Jia Binhua Hu Jing Wang Jianquan Liu Huanhuan Liu 

机构地区：[1]Key Laboratory for Bio-resources and Eco-environment&State Key Lab of Hydraulics&Mountain River Engineering,Sichuan Zoige Alpine Wetland Ecosystem National Observation and Research Station,Key Laboratory for Bio-resource and Eco-environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610065,China [2]Institute of Biotechnology and Nuclear Technology,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 610066,China [3]Chongqing Institute of Green and Intelligent Technology,Chinese Academy of Sciences,Chongqing,China

出　　处：《Molecular Plant》2025年第1期42-58,共17页分子植物(英文版)

基　　金：supported by the Natural Science Foundation of China(No.32270302 and No.32030006);the Fundamental Research Funds for the Central Universities(2020SCUNL207 and SCU2022D003);Natural Science Foundation of Sichuan Province(2024NSFSC0340 and 25QNJJ4460);Sichuan Forage Innovation Team Program(SCCXTD-2024-16).

摘　　要：Submergence stress tolerance is a complex trait governed by multiple loci.Because of its wide distribution across arid and humid regions,Arabidopsis thaliana offers an opportunity to explore the genetic components and their action mechanisms underlying plant adaptation to submergence stress.In this study,using map-based cloning we identified WRKY22 that activates RAP2.12,a locus previously identified to contribute to the submergence stress response,to regulate plant humid adaptation possibly through ethylene signal transduction in Arabidopsis.WRKY22 expression is inhibited by ARABIDOPSIS RESPONSE REGULATORs(ARRs)but activated by the WRKY70 transcription factor.In accessions from humid environments,a two-nucleotide deletion in the WRKY22 promoter region prevents binding of phosphorylated ARRs,thereby maintaining its high expression.Loss of the ARR-binding element in the WRKY22 promoter underwent strong positive selection during colonization of A.thaliana in the humid Yangtze River basin.However,the WRKY70-binding motif in the WRKY22 promoter shows no variation between accessions from humid and arid regions.These findings together establish a novel signaling axis wherein WRKY22 plays a key role in regulating the adaptive response that enables A.thaliana to colonize contrasting habitats.Notably,we further showed functional conservation of this locus in Brassica napus,suggesting that modulating this axis might be useful in the breeding of flood-tolerant crop varieties.

关 键 词：WRKY22 RAP2.12 WRKY70 ARRs natural variation submergence tolerance 

分 类 号：Q94[生物学—植物学]