基于HPLC指纹图谱结合化学计量学方法分析评价鸡血藤超微粉的质量  

Quality evaluation of ultrafine powder of Spatholobus suberectus based on HPLC fingerprint combined with stoichiometry methods

作　　者：吴国学[1] 杨书婷 刘栋 李小霞 董靖[1] 郝义彬 王一硕[3] WU Guoxue;YANG Shuting;LIU Dong;LI Xiaoxia;DONG Jing;HAO Yibin;WANG Yishuo(The Fifth Clinical Medical College of Henan University of Chinese Medicine/Zhengzhou People's Hospital,Zhengzhou 450003,China;Henan Provincial People's Hospital,Zhengzhou 450000,China;Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学第五临床医学院/郑州人民医院,郑州450003 [2]河南省人民医院,郑州450000 [3]河南中医药大学,郑州450046

出　　处：《军事医学》2024年第11期832-837,共6页Military Medical Sciences

基　　金：河南省中医药文化与管理研究项目重点课题(TCM2021002,TCM2023031);河南省医学科技攻关计划项目(20180208034,LHGJ20191089);河南省中医药科学研究专项课题(20-21ZY2141);国家中医临床研究基地科研专项课题(2019JDZX2123)。

摘　　要：目的建立不同来源鸡血藤超微粉的高效液相色谱(HPLC)指纹图谱,并结合多模式化学计量学分析评价其质量。方法采用Amethyst C_(18)-H色谱柱(4.6 mm×250 mm,5μm),以0.1%磷酸溶液(A)-乙腈(B)为流动相进行梯度洗脱,流速为1.0 mL/min,检测波长260 nm,柱温为30℃。并运用聚类分析、偏最小二乘法-判别分析和主成分分析对鸡血藤指纹图谱进行模式识别研究。结果10批次鸡血藤超微粉共标定13个共有峰,经对照品比对指认了3种成分,指纹图谱相似度范围为0.921~0.989,具有良好的相似性。聚类分析将样品分为3类,主成分分析用4个主成分进行评价,综合得分显示,S6、S1、S2、S3等产地的鸡血藤超微粉样品质量较好。偏最小二乘法‐判别分析筛选了不同产地鸡血藤超微粉的4个质量差异标志物,分别为表儿茶素、色谱峰1、色谱峰6及色谱峰4。结论所建立的指纹图谱方法简便、重复性好,结合多模式化学计量学分析可用于评价鸡血藤超微粉的质量。Objective To evaluate the quality of ultrafine powder of Spatholobus suberectus from different sources by combined HPLC fingerprint and chemical pattern recognition.Methods Separation was performed on a Amethyst C_(18)-H column(4.6 mm×250 mm,5μm)and the mobile phase was 0.1%phosphoric acid-acetonitrile with gradient elution.The flow rate was 1.0 mL/min and the wavelength was 260 nm.The column temperature was 30℃.The cluster analysis(CA),least partial squares-discriminant analysis(PLS-DA)and principal component analysis(PCA)were applied to studying the HPLC fingerprint and chemical pattern recognition.Results Thirteen common peaks were identified in ultrafine powder samples of 10 batches of Spatholobus suberectus and 3 components were identified by reference substances.The similarity of fingerprint was 0.921-0.989,indicating good similarity.The samples were divided into 3 groups by cluster analysis;principal component analysis results extracted 4 principal components.According to the composite score,the quality of ultrafine powder samples of Spatholobus suberectus from S6,S1,S2,S3 were better than others.And 4 kinds of marker compounds that caused the quality difference of ultrafine powder samples of Spatholobus suberectu were screened out through the least partial squares-discriminant analysis,which were epicatechin and peaks 1,6 and 4 respectively.Conclusion The fingerprint method is simple and reproducible,and can be used to evaluate the quality of ultrafine powder samples of Spatholobus suberectus in combination with multi-mode stoichiometric analysis.

关 键 词：鸡血藤 超微粉 指纹图谱 聚类分析 主成分分析 偏最小二乘法-判别分析 

分 类 号：R284.1[医药卫生—中药学]