链脲佐菌素和氢化可的松诱导大鼠慢性难愈性伤口模型的比较研究  

Comparative study on streptozotocin and hydrocortisone-induced chronic non-healing wound models in rats

作　　者：朱毅仁 陈品华 张正超 何雪义 王若礼 方琦 何武兵 Zhu Yiren;Chen Pinhua;Zhang Zhengchao;He Xueyi;Wang Ruoli;Fang Qi;He Wubing(Shengli Clinical Medical College of Fujian Medical University,Fujian,Fuzhou 350001,China;Department of Emergency Surgery,Fuzhou University Affiliated Provincial Hospital,Fujian,Fuzhou 350001,China;Fujian Trauma Medicine Center,Fujian Fuzhou 350001,China)

机构地区：[1]福建医科大学省立临床医学院,福建福州350001 [2]福州大学附属省立医院急诊外科,福建福州350001 [3]福建省创伤医学中心,福建福州350001

出　　处：《创伤与急诊电子杂志》2024年第4期237-243,共7页Journal of Trauma and Emergency(Electronic Version)

基　　金：福建省医学创新课题(2020CXA007);福建省自然科学基金(2024J011027)。

摘　　要：目的比较链脲佐菌素(streptozotocin,STZ)与氢化可的松诱导的两种慢性难愈性伤口模型的特征及其应用价值。方法将36只SD大鼠采用随机数字表法分为对照组、糖尿病组(STZ诱导)和激素组(氢化可的松诱导),每组12只。观察并记录各组大鼠体重、血糖,在各组大鼠背部制作标准全层皮肤缺损创面,连续观察14d并记录伤口愈合过程,比较创面面积变化及愈合情况。结果体重方面,自诱导第2周开始,对照组[(212.83±8.52)g]和激素组[(213.67±12.75)g]体重显著高于糖尿病组[(198.00±5.17)g],差异有统计学意义(P<0.05)。血糖水平方面,在糖尿病组注射STZ后第1周起,糖尿病组出现显著血糖升高,12只大鼠随机血糖均>16.8mmol/L,而对照组和激素组血糖水平始终保持正常。创面愈合方面,以创伤后即刻创面面积为基准值(100%),对照组愈合最快,创伤后第1、3、7、10和14天的创面面积百分比分别为(79.00±3.11)%、(61.75±3.94)%、(28.81±2.38)%、(16.22±3.87)%和(3.25±3.75)%;糖尿病组创面愈合明显延迟,第14天时仍有显著创面,表现为创面干燥、微循环障碍、肉芽组织形成不良,相应时间点的创面面积百分比分别为(97.12±3.23)%、(96.35±3.05)%、(85.43±2.13)%、(65.02±1.63)%和(43.54±2.56)%,显著大于对照组(P<0.001);激素组除表现为创面渗出增多、肉芽组织形成受抑制外,特征性地在第7~10天期间出现创面显著恶化,各时间点创面面积百分比分别为(93.96±4.13)%、(87.10±4.24)%、(63.99±5.01)%、(100.04±5.69)%和(101.16±6.26)%。3组间创面面积百分比差异具有统计学意义(P<0.001)。结论STZ诱导的大鼠糖尿病模型和氢化可的松诱导的大鼠激素模型均可成功构建慢性难愈性伤口,但具有不同的病理特征和局限性。大鼠糖尿病模型呈现相对稳定的持续性愈合延迟,而大鼠激素模型虽然具有特征性的创面恶化期,但个体差异大,难以建立统一的评估标准。因此,�Objective To compare the characteristics and application value of two chronic non-healing wound models induced by streptozotocin(STZ)and hydrocortisone in rats.Method Thirty-six SD rats were randomly divided into control group,diabetic group induced by STZ,and hormonal group induced by hydrocortisone,with 12 rats in each group.Body weight and blood glucose were observed and recorded.Standard full-thickness skin defect were created on the rats'backs,and wound healing was continuously monitored for 14 days.Changes in wound area and the progression of healing were compared throughout the study.Result From the second week onwards,the control group[(212.83±8.52)]g and hormonal group[(213.67±12.75)g]showed significantly higher body weights compared to the diabetic group[(198.00±5.17)g,P<0.05].Blood glucose levels in the diabetic group became significantly elevated from the first week after STZ injection,with random blood glucose levels exceeding 16.8 mmol/L in all 12 rats,while the control and hormonal groups maintained normal blood glucose levels.Wound healing was assessed using the immediate post-injury wound area as the baseline(100%).The control group demonstrated the fastest healing,showing wound area percentages of(79.00±3.11)%,(61.75±3.84)%,(28.81±2.38)%,(16.22±3.87)%,and(3.25±3.75)%on post-injury day 1,3,7,10,and 14,respectively.The diabetic group showed significantly delayed wound healing,with substantial wound areas persisting on day 14,characterized by dry wounds,microcirculatory disorders,and poor granulation tissue formation.The corresponding wound area percentages were(97.12±3.23)%,(96.35±3.05)%,(85.43±2.13)%,(65.02±1.63)%,and(43.54±2.56)%,which were significantly larger than those in the control group(P<0.001).The hormonal group exhibited increased wound exudation and suppressed granulation tissue formation,characterized by significant wound deterioration between days 7-10.Wound area percentages at different time points were(93.96±4.13)%,(87.10±4.24)%,(63.99±5.01)%,(100.04±5.69)%,and(101

关 键 词：慢性难愈性伤口 糖尿病 氢化可的松 动物模型 伤口愈合 

分 类 号：R58[医药卫生—内分泌]