江苏省、山东省和安徽省禽致病性大肠杆菌的分子流行病学调查  

Molecular Epidemiological Investigation of Avian Pathogenic Escherichia coli in Jiangsu,Shandong,and Anhui Province

作　　者：邵立志 吉星 孙利厂[2] 朱树娇 王佳芸 何涛[2] 王冉[2] 杨章平[1] SHAO Lizhi;JI Xing;SUN Lichang;ZHU Shujiao;WANG Jiayun;HE Tao;WANG Ran;YANG Zhangping(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China;Jiangsu Academy of Agricultural Sciences-Institute of Agricultural Quality,Safety and Nutrition Jiangsu Provincial Key Laboratory of Food Quality and Safety-Provincial-Ministerial Cultivation Base of National Key Laboratory,Nanjing 210014,China)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省农业科学院农产品质量安全与营养研究所、省部共建国家重点实验室培育基地、江苏省食品质量安全重点实验室,江苏南京210014

出　　处：《中国兽医杂志》2025年第2期1-9,共9页Chinese Journal of Veterinary Medicine

基　　金：国家重点研发计划(2022YFD1800400)。

摘　　要：为了探明江苏省、山东省和安徽省鸡源禽致病性大肠杆菌(APEC)的分子流行病学特征,本试验于2023年从三省部分规模化鸡养殖场采集鸡粪便、鸡毛蛋、鸡卵黄以及病死鸡的鸡腿、骨髓、关节、心脏、脾脏、肺脏和肠道内容物样品共918份,使用选择性培养基分离APEC;利用微量肉汤稀释法测定分离菌株对常用抗菌药物的敏感性;基于全基因组测序分析分离菌株的耐药基因和毒力基因携带情况;通过多位点序列分型(MLST分型)、菌体(O)抗原分型和系统发育树分析分离菌株的主要流行特征。结果显示,共分离获得390株APEC,分离率为42.48%;分离菌株对氨苄西林(91.03%,355/390)、头孢噻呋(82.82%,323/390)、庆大霉素(81.03%,316/390)和四环素(77.95%,304/390)的耐药率较高,对美罗培南(0.51%,2/390)和黏菌素(3.08%,12/390)比较敏感,耐药基因以blaEC(98.97%,386/390)、blaTEM(80.00%,312/390)、aph(6)-Id(54.10%,211/390)、erm(D)(98.46%,384/390)、tet(A)(81.79%,319/390)和floR(63.33%,247/390)为主;毒力基因分析发现,所有分离菌株至少含有4种毒力基因。共鉴定出68种血清型,其中优势血清型为O78(11.03%,43/390)、O180(7.95%,31/390)和O15(7.18%,28/390),MLST共鉴定出71种ST型,主要的ST型为ST117(15.90%,62/390)、ST155(8.46%,33/390)和ST162(8.21%,32/390);系统发育树结果显示,分离菌株共分为4个进化分支,不同省份鸡养殖场分离菌株之间的遗传进化关系相距较远。结果表明,江苏省、山东省和安徽省鸡源APEC的流行血清型为O78、O180和O15,主要流行ST型为ST117,携带的耐药基因和毒力基因较多,鸡养殖场应当继续加强APEC的流行病学监测。To elucidate the molecular epidemiological characteristics of avian pathogenic Escherichia coli(APEC)in Jiangsu,Shandong,and Anhui provinces,this study collected 918 samples in 2023 from large-scale poultry farms.Samples included chicken feces,feather-deposited eggs,yolks,and thighs,bone marrow,joints,hearts,spleens,lungs,and intestinal contents from diseased or deceased chickens.APEC strains were isolated using selective media,and antimicrobial susceptibility was assessed via broth microdilution.Whole-genome sequencing was employed to analyze resistance and virulence genes,and multilocus sequence typing(MLST),O-antigen serotyping,and phylogenetic tree construction were performed to determine the epidemiological characteristics of the isolates.A total of 390 APEC strains were isolated,with an isolation rate of 42.48%.The isolates exhibited high resistance rates to ampicillin(91.03%,355/390),ceftiofur(82.82%,323/390),gentamicin(81.03%,316/390),and tetracycline(77.95%,304/390),and were sensitive to meropenem(0.51%,2/390)and colistin(3.08%,12/390)Predominant resistance genes included blaEC(98.97%,386/390),blaTEM(80.00%,312/390),aph(6)-Id(54.10%,211/390),erm(D)(98.46%,384/390),tet(A)(81.79%,319/390),and floR(63.33%,247/390).Virulence gene analysis revealed that all isolates carried at least four virulence genes.A total of 68 serotypes were identified,with the predominant serotypes being O78(11.03%,43/390),O180(7.95%,31/390),and O15(7.18%,28/390).A total of 71 sequence types(STs)were identified through MLST,with the main ST types being ST117(15.9%,62/390),ST155(8.46%,33/390),and ST162(8.2%,32/390).Phylogenetic analysis showed that isolates formed four evolutionary clades,with substantial genetic divergence among isolates from different provinces.These findings indicate that the predominant APEC serotypes in Jiangsu,Shandong,and Anhui provinces are O78,O180,and O15,with ST117 being the primary sequence type.The isolates exhibited high prevalence of resistance and virulence genes.Continued epidemiological monitoring of

关 键 词：禽致病性大肠杆菌(APEC) 耐药基因 毒力基因 血清型 流行病学调查 

分 类 号：S858.3[农业科学—临床兽医学] S855.1[农业科学—兽医学]