表皮葡萄球菌多糖与牛支原体P48蛋白偶联物的免疫效力初步研究  

Preliminary Study on the Immunogenicity of Staphylococcus epidermidis Polysaccharide-Mycoplasma bovis P48 Protein Conjugate

作　　者：刘国瑞 田欣睿 吴浩 吴文学[2] 李秀波[3] 李存 LIU Guorui;TIAN Xinrui;WU Hao;WU Wenxue;LI Xiubo;LI Cun(College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China;College of Veterinary Medicine,China Agricultural University,Beijing 100193,China;Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]天津农学院动物科学与动物医学学院,天津西青300384 [2]中国农业大学动物医学院,北京海淀100193 [3]中国农业科学院饲料研究所,北京海淀100081

出　　处：《中国兽医杂志》2025年第2期25-32,共8页Chinese Journal of Veterinary Medicine

基　　金：国家现代农业产业技术体系(CARS36)。

摘　　要：为评价表皮葡萄球菌多糖(PS)和牛支原体P48蛋白的偶联物PS-P48在小鼠上的安全性和免疫效力,本试验将20只BALB/c小鼠随机均分为4个组,对照组、多糖组、蛋白组和偶联组,分别接种0.9%生理盐水、PS、P48蛋白和偶联物PSP48,每隔14 d免疫1次,共免疫3次;分别于免疫0、7、14、21、28、35、42、49和56 d进行临床观察和称重,采集血清进行抗体效价检测和细胞因子检测。结果显示,免疫全过程未出现小鼠死亡个例,各组小鼠精神状态良好,无不良反应;免疫35、42、49和56 d时,多糖组小鼠体重显著低于对照组(P<0.05或P<0.01);免疫42 d时,蛋白组小鼠体重显著低于对照组(P<0.05);免疫42、49和56 d时,偶联组小鼠体重显著低于对照组(P<0.05或P<0.01);从抗体水平看,免疫后的各个采样时间点,偶联组、蛋白组和多糖组小鼠血清IgG抗体平均P/N值均极显著高于对照组(P<0.01),偶联组和蛋白组抗体平均P/N值均极显著高于多糖组(P<0.01);蛋白组和偶联组小鼠血清IgG抗体平均P/N值在免疫49 d时达到峰值,偶联组针对P48蛋白的抗体平均P/N值极显著高于蛋白组(P<0.01),蛋白组的抗体效价达1∶25600,偶联组的抗体效价>1∶51200;且偶联组中针对表皮葡萄球菌PS的抗体效价达1∶800,针对牛支原体P48蛋白的抗体效价>1∶6400;在免疫56 d时,偶联组免疫小鼠血清中IL-4、IL-6和IFN-γ水平显著高于蛋白组、多糖组和对照组(P<0.05或P<0.01)。结果表明,偶联物PS-P48可同时产生针对表皮葡萄球菌PS和牛支原体P48蛋白的抗体,能够诱导特异性抗体产生,并激活细胞因子表达,为进一步研究和发展同时针对表皮葡萄球菌和牛支原体的疫苗,尤其是用于奶牛乳房炎的疫苗提供了重要的参考。This study aimed to evaluate the safety and immunogenicity of the polysaccharide(PS)of Staphylococcus epidermidis conjugated with the P48 protein of Mycoplasma bovis(PS-P48)in mice.Twenty BALB/c mice were randomly divided into four groups:Control,Polysaccharide,Protein,and Conjugate groups.Mice were immunized with 0.9%saline,PS,P48 protein,or PSP48 conjugate,respectively,at 14-day intervals for three rounds of immunization.Clinical observations and body weight measurements were conducted on days 0,7,14,21,28,35,42,49,and 56 post-immunization.Serum samples were collected for antibody titration and cytokine analysis.The results showed no mortality or adverse reactions throughout the immunization period,with all groups maintaining good physical conditions.At 35,42,49,and 56 days post-immunization,the body weight of the Polysaccharide group was significantly lower than that of the Control group(P<0.05 or P<0.01).At 42 days,the body weight of the Protein group was significantly lower than that of the Control group(P<0.05).At 42,49,and 56 days,the body weight of the Conjugate group was significantly lower than that of the Control group(P<0.05 or P<0.01).In terms of antibody levels,the mean serum IgG P/N values of the Conjugate,Protein,and Polysaccharide groups were significantly higher than those of the Control group(P<0.01)at all time points post-immunization.The mean P/N values of the Conjugate and Protein groups were significantly higher than those of the Polysaccharide group(P<0.01).The mean P/N values of the Protein and Conjugate groups peaked at 49 days post-immunization.The Conjugate group's mean P/N value against the P48 protein was significantly higher than that of the Protein group(P<0.01),with antibody titers reaching 1∶25600 in the Protein group and exceeding 1∶51200 in the Conjugate group.Additionally,the antibody titer against PS in the Conjugate group was 1∶800,while the titer against P48 exceeded 1∶6400.At 56 days post-immunization,serum IL-4,IL-6,and IFN-γlevels in the Conjugate group were sig

关 键 词：奶牛乳房炎 表皮葡萄球菌 多糖 牛支原体 偶联物PS-P48 

分 类 号：S562.61[农业科学—作物学]