基于网络药理学探讨小儿定喘口服液治疗儿童哮喘的作用机制  

Mechanism of Xiao’er Dingchuan Oral Liquid in the Treatment of Children with Asthma Based on Network Pharmacology

作　　者：郝梦缘 林荣军[1] 刘冬云[1] 韩振靓[1] 路玲[1] 金蓉[1] Hao Mengyuan;Lin Rongjun;Liu Dongyun;Han Zhenliang;Lu Ling;Jin Rong(Qingdao University Affiliated Hospital,Shandong Qingdao 266000,China)

机构地区：[1]青岛大学附属医院,山东青岛266000

出　　处：《儿科药学杂志》2025年第2期5-11,共7页Journal of Pediatric Pharmacy

基　　金：国家自然科学基金项目,编号81701587;山东省中医药科技项目,编号M-2022227。

摘　　要：目的:探索小儿定喘口服液治疗儿童哮喘的成分、靶点信息及网络药理学机制。方法:依次借助中药系统药理学数据库与分析平台(TCMSP)、PubChem、SwissTargetPrediction数据库预测小儿定喘口服液成分和靶点,综合在线人类孟德尔遗传数据系统(OMIM)、GeneCards数据库获取儿童哮喘相关疾病靶点。在微生信平台绘制韦恩图,输出药物和疾病的共同靶点,借助CytoScape软件构建“药物-成分-靶点”网络,筛选出核心成分。联合STRING数据库、CytoNCA工具进行蛋白相互作用(PPI)分析,筛选出核心靶点。运用DAVID数据库对共同靶点进行基因本体(GO)和京都基因与基因组百科全书(KEGG)通路富集分析。结果:筛选出小儿定喘口服液172个活性成分,931个靶点,2787个儿童哮喘靶点,光甘草定、黄芩素、汉黄芩素等10个核心成分及表皮生长因子受体(EGFR)、白细胞介素-6(IL-6)、信号转导和转录激活因子3(STAT3)等10个核心靶点。KEGG富集通路主要涉及186条,包括磷脂酰肌醇3激酶(PI3K)-蛋白激酶B(Akt)、丝列原活化蛋白激酶(MAPK)、钙离子等信号通路。结论:小儿定喘口服液治疗儿童哮喘通过多成分、多靶点、多途径发挥作用。Objective:To explore the components,target information and network pharmacological mechanism of Xiao’er Dingchuan oral liquid in the treatment of children with asthma.Methods:Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),PubChem and SwissTargetPrediction databases were retrieved to predict the components and targets of Xiao’er Dingchuan oral liquid.On-line Mendelian Inheritance in Man(OMIM)and GeneCards databases were retrieved to obtain the asthma-related disease targets.Venn diagram was drawn on bioinformatics platform to output the common targets of drugs and diseases,and the“drug-component-target”network was constructed with CytoScape software to screen out the core components.Protein-protein interaction analysis was carried out in combination with STRING database and CytoNCA tool to screen out core targets.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis were performed for common targets by using DAVID database.Results:Totally 172 active components,931 targets,2,787 children’s asthma targets,10 core components including glabridin,baicalin and wogonin,as well as 10 core targets including epidermal growth factor receptor(EGFR),interleukin-6(IL-6),and signal transduction and activator of transcription 3(STAT3)were screened out from Xiao’er Dingchuan oral liquid.There were 186 KEGG enrichment pathways,including phosphatidylinositol 3-kinase-protein kinase B(PI3K-Akt),mitogen activated protein kinase(MAPK),calcium ion and other signaling pathways.Conclusion:Xiao’er Dingchuan oral liquid plays an important role in the treatment of children with asthma through multi-components,multi-targets and multi-pathways.

关 键 词：小儿定喘口服液 儿童 哮喘 网络药理学 作用机制 

分 类 号：R725.1[医药卫生—儿科]