基于UHPLC-Q-Exactive Orbitrap MS技术的天山雪莲口服液化学成分快速表征  

Rapid Identification of Chemical Constituents in Sasussured Involucrata Oral Liquid Based on UHPLC-Q-Exactive Orbitrap MS Technology

作　　者：李凯琳 刘玲 邹宇凤 陈逸凡 唐才俊 杨建波[3,4] LI Kailin;LIU Ling;ZOU Yufeng;CHEN Yifan;TANG Caijun;YANG Jianbo(School of Pharmacy,Shandong Second Medical University,Weifang 261053,China;School of Pharmaceutical Sciences,Hunan University of Medicine,Huaihua 418000,China;Institute for Control of Chinese Traditional Medicine and Ethnic Medicine,National Institutes for Food and Drug Control,Beijing 100050,China;NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine(Uyghur)Medicine,Xinjiang Uygur Autonomous Region Drug Inspection and Research Institute,Urumqi 830054,China)

机构地区：[1]山东第二医科大学药学院,山东潍坊261053 [2]湖南医药学院药学院,湖南怀化418000 [3]中国食品药品检定研究院中药民族药检定所,北京100050 [4]新疆维吾尔自治区药品检验研究院,国家药品监督管理局中药(维药)质量控制重点实验室,新疆乌鲁木齐830054

出　　处：《中国现代中药》2025年第2期210-218,共9页Modern Chinese Medicine

基　　金：新疆维吾尔自治区智力援疆创新拓展人才计划项目。

摘　　要：目的:利用超高效液相色谱-四级杆-静电场轨道阱质谱法(UHPLC-Q-Exactive Orbitrap MS)鉴定天山雪莲口服液主要化学成分,明确其物质基础。方法:采用Waters ACQUITY UPLC BEH C18色谱柱(100 mm×2.1 mm,1.7μm),以流动相0.1%甲酸水溶液(A)-乙腈(B)进行梯度洗脱,电喷雾离子源,正、负离子监测模式。结果:通过对照品、数据库和参考文献比对,共鉴定了48个化学成分,其中44个是首次从天山雪莲口服液中发现,新绿原酸、芦丁、金丝桃苷等18个化合物为准确鉴定。结论:该方法可以快速有效地鉴定天山雪莲口服液中的主要化学成分,为其质量控制、有效成分和作用机制研究提供参考。Objective:To identify the main chemical constituents of Sasussured involucrata oral liquid by using UHPLC-Q-Exactive Orbitrap MS technology and clarify the material basis.Methods:Waters ACQUITY UPLC BEH C18 chromatographic column(100 mm×2.1 mm,1.7µm)was used,with 0.1%formic acid(A)-acetonitrile(B)as mobile phase for gradient elution.An electrospray ionization(ESI)source was used and operated in both positive and negative ion modes.Results:A total of 48 chemical constituents were identified by comparing the reference standards,databases,and bibliography data,44 of those were reported in Sasussured involucrata oral liquid for the first time,and 18 chemical constituents were accurately identified.Conclusion:The method can rapidly and effectively identify the main chemical constituents of Sasussured involucrata oral liquid,laying a foundation for research on its quality control,effective components,and mechanism of action.

关 键 词：天山雪莲 化学成分 超高效液相色谱-四级杆-静电场轨道阱质谱法 

分 类 号：R284[医药卫生—中药学]