基于DSS标记鉴别牡丹、芍药及川赤芍  

Identification of Paeonia suffruticosa,Paeonia lactiflora,and Paeonia veitchii Based on DNA Signature Sequence Tags

作　　者：陈灵丽 王多梅 蒲婧哲 胡冲 蒋超[3] 袁媛[4] 张亚中 CHEN Lingli;WANG Duomei;PU Jingzhe;HU Chong;JIANG Chao;YUAN Yuan;ZHANG Yazhong(Anhui Institute of Food and Drug Control,Drug Inspection and Research Institute/NMPA Key Laboratory for Quality Research and Evaluation of Traditional Chinese Medicine,Hefei 230051,China;Huainan Municipal Food and Drug Inspection Center,Huainan 232000,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Experimental Research Center,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]安徽省食品药品检验研究院药品检验研究所/国家药品监督管理局中药质量研究与评价重点实验室,安徽合肥230051 [2]淮南市食品药品检验中心,安徽淮南232000 [3]中国中医科学院中药资源中心,道地药材品质保障与资源持续利用全国重点实验室,北京100700 [4]中国中医科学院医学实验中心,北京100700

出　　处：《中国现代中药》2025年第2期242-247,共6页Modern Chinese Medicine

基　　金：国家药品监督管理局中成药质量评价重点实验室开放课题(2024-KFKT-002);安徽省药品监督管理局监管科学研究重点项目(AHYJ-KJ-202308);安徽省药品监督管理局科技创新项目(AHYJ-KJ-202313)。

摘　　要：目的:建立一种快速、简便、准确鉴别牡丹、芍药及川赤芍的DNA特征序列(DSS)标记分子鉴定方法。方法:基于芍药属植物叶绿体基因组数据库,采用IdenDSS软件筛选出DSS组及其特异性引物对,经DNA提取、聚合酶链式反应扩增、测序、序列比对进行牡丹、芍药及川赤芍目标DSS验证。结果:通过对5对候选DSS的验证,确定联合2个DSS(GTAACTTGCTATCCTCTTGCCTAGCAGGCAAAGATTTACT、ACTACGAA GAAGTTCCGGGGGTTACAAAGGAAACTTCGAG)标记可用于牡丹、芍药及川赤芍的鉴定,将DSS上传至中药分子鉴定平台(http://www. herbsdna. com)标准数据库中,结合数据库的BLAST功能,可实现牡丹、芍药及川赤芍的快速、准确鉴定。结论:筛选的DSS标记可用于牡丹皮、白芍及赤芍的基原鉴定。Objective:To establish a quick,convenient,and accurate DNA signature sequence tag(DSS)-based molecular identification method to identify Paeonia suffruticosa,P.lactiflora,and P.veitchii.Methods:DSS groups and their specific primers were screened by using IdenDSS software based on the chloroplast genome database of plants in Paeonia L..After DNA extraction,polymerase chain reaction(PCR)amplification,sequencing,and sequence alignment,the target DSSs used to validate P.suffruticosa,P.lactiflora,and P.veitchii were obtained.Results:The verification results of five candidate DSSs determined that two DSSs(GTAACTTGCTATCCTCTTGCCTAGCAGGCAAAGATTTACT and ACTACGAAGAAGTTCCGGGGGTTACAAAGGAAACTTCGAG)could be used for the identification of P.suffruticosa,P.lactiflora,and P.veitchii.The DSSs were uploaded to the Traditional Chinese Medicine Molecular Identification Platform(http://www.herbsdna.com).With the help of the BLAST function,a rapid and accurate identification of P.suffruticosa,P.lactiflora,and P.veitchii was achieved.Conclusion:The screened DSSs could be effectively applied to the origin identification of P.suffruticosa,P.lactiflora,and P.veitchii.

关 键 词：牡丹 芍药 川赤芍 DNA特征序列 基原鉴定 

分 类 号：R282.5[医药卫生—中药学]