基于谱效关联-网络药理学探讨青钱柳抗糖尿病的功能成分及作用机制  

Exploration of Functional Components and Antidiabetic Mechanism of Cyclocarya Paliurus Based on Spectrum-effect Relationship and Network Pharmacology

作　　者：杨乐乐 高昊[1] 倪静云 YANG Lele;GAO Hao;NI Jingyun(Institute of Traditional Chinese Medicine and Natural Products,College of Pharmacy,Jinan University,Guangzhou 510632,China;Zhuhai Hengqin Aoye Health Technology Co.,Ltd.,Zhuhai 519031,China)

机构地区：[1]暨南大学药学院中药及天然药物研究所,广东广州510632 [2]珠海横琴澳叶健康科技有限公司,广东珠海519031

出　　处：《中国现代中药》2025年第2期274-281,共8页Modern Chinese Medicine

基　　金：国家自然科学基金项目(82204588)。

摘　　要：目的:基于谱效关联-网络药理学筛选青钱柳抗糖尿病的功能成分及作用机制。方法:首先采用超高效液相色谱-质谱法(UPLC-MS)对青钱柳提取物进行化学成分的定性及定量表征。随后,检测不同批次样品对细胞糖消耗的影响。将化学成分定量信息与糖消耗活性数据进行谱效关联分析,根据变量重要性投影(VIP)值和r,同时结合定性分析,筛选潜在活性成分。最后,借助网络药理学,进一步阐明青钱柳抗糖尿病的作用机制。结果:构建10批青钱柳的UPLC-MS指纹图谱,比较这些样品的葡萄糖消耗差异。青钱柳UPLC-MS指纹图谱与药效进行关联,筛选出VIP值>1.2且与活性呈正相关的潜在质谱峰。通过质谱定性数据鉴定出5个化学成分,包括绿原酸、山柰酚、槲皮素-3-葡萄糖醛酸苷、杨梅素-3-葡萄糖苷、异槲皮苷。最后,结合网络药理学,阐明了青钱柳通过多成分、多靶点发挥抗糖尿病的药理作用机制。结论:建立的“谱-效-靶”策略能够比较系统地对青钱柳的复杂化学成分进行全面分析,有效揭示其发挥抗糖尿病作用的药效功能成分和作用机制,为青钱柳的临床研究提供参考。Objective:This study aims to explore the functional components and antidiabetic mechanism of Cyclocarya Paliurus based on the spectrum-effect relationship and network pharmacology.Methods:Firstly,ultra-performance liquid chromatography-mass spectrometry(UPLC-MS)was used to qualitatively and quantitatively characterize the chemical components of C.paliurus extracts.Secondly,the effect of different batches of samples on cellular glucose consumption was examined.Spectrum-effect relationship analysis was performed between quantitative information of chemical components and glucose consumption activity data,and potential active components were screened according to variable importance projection(VIP)value and correlation coefficient combined with qualitative analysis.Finally,network pharmacology was further utilized to clarify the antidiabetic mechanism of C.paliurus.Results:The fingerprints of 10 batches of C.paliurus samples were constructed,and the difference in glucose consumption of these samples was compared.Potential mass spectral peaks with VIP>1.2 and positive correlation with activity were screened according to the correlation between the UPLC-MS fingerprint and drug efficacy.Five components were identified using qualitative data by mass spectrometry,including chlorogenic acid,kaempferol,quercetin 3-O-β-D-glucuronide,myricetin-3-glucopyranoside,and isoquercetin.Finally,the antidiabetic mechanism of C.paliurus through multiple components and targets was clarified by network pharmacology.Conclusion:The"spectrum-efficacy-target"strategy proposed in this paper provides a comprehensive analysis of the chemical components in C.paliurus,effectively revealing the antidiabetic functional components and the underlying mechanisms.The results can provide a reference for clinical research on C.paliurus.

关 键 词：青钱柳 糖尿病 谱效关系 高分辨质谱 功能成分 网络药理学 

分 类 号：R282[医药卫生—中药学]