谷子SHMT基因家族全基因组鉴定与表达分析  

Genome-wide identification and expression analysis of SHMT gene family in foxtail millet(Setaria italica L.)

作　　者：郭冰 秦家范 李娜[1] 宋梦瑶 王黎明[1] 李君霞[2] 马小倩 GUO Bing;QIN Jia-Fan;LI Na;SONG Meng-Yao;WANG Li-Ming;LI Jun-Xia;MA Xiao-Qian(College of Agriculture,Henan University of Science and Technology,Luoyang 471023,Henan,China;Cereal Crops Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;Luoyang Academy of Agricultural and Forestry Sciences,Luoyang 471023,Henan,China)

机构地区：[1]河南科技大学农学院,河南洛阳471023 [2]河南省农业科学院粮食作物研究所,河南郑州450002 [3]洛阳市农林科学院,河南洛阳471023

出　　处：《作物学报》2025年第3期586-597,共12页Acta Agronomica Sinica

基　　金：河南省重点研发专项(231111110300);国家自然科学基金项目(32301841);河南省自然科学基金项目(242300421320);河南科技大学A类博士人才项目(13480103)资助。

摘　　要：丝氨酸羟甲基转移酶(SHMT)在作物中广泛存在,参与作物碳代谢、光呼吸等途径,在作物生长发育及抗逆胁迫中发挥重要作用,但是目前关于谷子SHMT基因的研究较少。本研究在全基因组水平鉴定了谷子SHMT基因家族成员,系统分析了SiSHMTs的基因结构、进化关系、染色体定位、物种间共线性、顺式作用元件、表达模式、优势单倍型等。结果表明,谷子中共有5个SiSHMTs,分子量在51.70~64.37 kD之间,空间结构相近,进化关系将其分为3组,每组成员散落在各条染色体上。启动子顺式作用元件分析发现,在启动子中包含多个光反应响应元件、厌氧响应元件、激素响应元件等作用元件。物种间共线性分析结果表明,SiSHMT3和SiSHMT4与单子叶作物水稻、小麦、高粱、玉米存在共线性,SiSHMT3与水稻、小麦、玉米存在多个共线对。SiSHMT成员在谷子不同发育时期、不同组织中表达量不同,SiSHMT4表达量较高,在穗发育过程持续高表达,并受到干旱、盐和ABA的显著诱导。SiSHMT4单倍型分析显示,Hap1为优势单倍型,在穗长、穗宽、穗重各表型中都显著优于其他单倍型。研究结果为挖掘谷子抗旱、耐盐新功能基因提供基因源,为今后选择培育高产抗逆谷子新品种奠定理论基础。Serine hydroxymethyltransferase(SHMT)is involved in carbon metabolism and photorespiration,and is widely present in crops,playing a critical role in growth,development,and stress resistance.However,the SHMT genes in foxtail millet are largely unexplored.In this study,we identified the members of the SiSHMT gene family at the whole-genome level and systematically analyzed their gene structures,evolutionary relationships,chromosomal localizations,interspecies collinearity,cis-acting elements,expression patterns,and dominant haplotypes.Our results revealed five SiSHMT members in foxtail millet,with molecular weights ranging from 51.70 to 64.37 kD,and similar spatial structures.Phylogenetic analysis classified these genes into three groups,with members distributed across different chromosomes.The analysis of cis-acting elements in the gene promoters indicated the presence of numerous photo-responsive elements,anaerobic response elements,hormone response elements,and other cis-acting elements.Interspecies collinearity analysis showed that SiSHMT3 and SiSHMT4 exhibited collinearity with their orthologous genes in monocot crops such as rice,wheat,sorghum,and maize,with SiSHMT3 displaying multiple collinear pairs with rice,wheat,and maize.The expression levels of SiSHMT family members varied across different developmental stages and tissues of foxtail millet.Notably,SiSHMT4 was highly expressed in developing panicles and was significantly induced by drought,salt,and ABA treatments.Haplotype analysis of SiSHMT4 revealed that Hap1 was the dominant haplotype,significantly outperforming other haplotypes in panicle length,width,and weight.These findings provide valuable gene resources for improving drought and salt tolerance in foxtail millet and lay a theoretical foundation for the breeding of high-yield,stress-resistant foxtail millet varieties in the future.

关 键 词：谷子 SHMTs 基因家族 生长发育 非生物胁迫 

分 类 号：S515[农业科学—作物学]