芥菜型油菜响应菌核病侵染表达特性与高抗性关联基因分析  

Analysis of genes associated with expression characteristics and high resistance in response to Sclerotinia sclerotiorum infection in Brassica juncea

作　　者：张金泽 周庆国 杨旭 王倩 肖莉晶 金海润 欧阳青静 余坤江 田恩堂[1] ZHANG Jin-Ze;ZHOU Qing-Guo;YANG Xu;WANG Qian;XIAO Li-Jing;JIN Hai-Run;OU-YANG Qing-Jing;YU Kun-Jiang;TIAN En-Tang(College of Agriculture,Guizhou University,Guiyang 550025,Guizhou,China;Anhui Dingke Seed Industry Co.,Ltd.,Hefei 230001,Anhui,China)

机构地区：[1]贵州大学农学院,贵州贵阳550025 [2]安徽鼎科种业有限责任公司,安徽合肥230001

出　　处：《作物学报》2025年第3期621-631,共11页Acta Agronomica Sinica

基　　金：贵州省科技支撑计划项目(黔科合支撑[2022]重点031);国家自然科学基金项目(32160483,32360497);贵州大学国家自然科学基金项目([2023]093号);贵州省粮油作物分子育种重点实验室项目(黔科合中引地[2023]008);贵州省高等学校功能农业重点实验室项目(黔教技[2023]007号)资助。

摘　　要：菌核病是油菜的主要病害,侵染后能导致油菜大幅减产。本研究对200份芥菜型油菜株系进行了菌核病抗性鉴定,并从中筛选出部分高抗性育种材料。然后选取高抗株系G21-243-1(HR)和感病株系G21-149-2(LR)作为研究材料,通过实验室模拟核盘菌侵染12 h、24 h、36 h后的叶片为材料进行转录组分析(RNA-Seq),共获得138.16 Gb的Clean Data。以菌核病感病株系为对照,以接种菌核病的同时期抗病株系为处理组,进行差异基因表达分析后,共检测到1899个上调表达基因,1330个下调基因表达,在2个时期同时检测到445个差异表达基因,在3个时期同时检测到90个差异表达基因。对全部差异表达基因进行GO和KEGG功能分析,在KEGG分析中显著富集的通路为Plant-pathogen interactions、Plant hormone signaling、MAPK signaling pathways-plants。结合转录组分析检测到的DEG及功能分析结果,初步筛选出20个菌核病抗性相关的候选基因,并随机选取其中6个候选基因进行了qRT-PCR分析。本研究的开展可为解析菌核病侵染寄生植物基因表达特性、菌核病抗性基因筛选及油菜的菌核病抗性育种奠定基础。Sclerotinia sclerotiorum is a major disease affecting rapeseed,often causing significant yield losses after infection.In this study,we evaluated the resistance of 200 Brassica juncea lines to S.sclerotiorum and selected one highly resistant line(G21-243-1,HR)and one susceptible line(G21-149-2,LR)for transcriptome analysis.A total of 138.16 Gb of clean data was generated by simulating leaf responses at 12,24,and 36 hours post-infection(hpi)with S.sclerotiorum.The analysis identified 1899 up-regulated genes and 1330 down-regulated genes,with 445 differentially expressed genes(DEGs)detected across two time points and 90 DEGs across all three time points.Subsequent Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional analyses revealed significant enrichment in pathways related to plant-pathogen interactions,plant hormone signaling,and the MAPK signaling pathway in plants.By integrating transcriptome and functional analysis results,we preliminarily identified 20 candidate genes associated with resistance to S.sclerotiorum.Six of these genes were randomly selected for validation via qRT-PCR analysis.The findings of this study provide a foundation for future research on S.sclerotiorum,including the characterization of gene expression,the identification of resistance genes,and the development of resistant rapeseed varieties.

关 键 词：芥菜型油菜 菌核病 转录组分析 差异表达基因 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]