小麦TaSnRK基因家族鉴定及在局部根区干旱下的表达分析  

Identification of TaSnRK gene family and expression analysis under localized root zone drought in wheat

作　　者：张恒 冯雅岚 田文仲 郭彬彬 张均[1] 马超[1] ZHANG Heng;FENG Ya-Lan;TIAN Wen-Zhong;GUO Bin-Bin;ZHANG Jun;MA Chao(Agronomy College,Henan University of Science and Technology,Luoyang 471000,Henan,China;College of Life Science,Wuchang University of Technology,Wuhan 430223,Hubei,China;Luoyang Academy of Agriculture and Forestry Sciences/Luoyang Dryland Agricultural Experimental Base,Chinese Academy of Agricultural Sciences,Luoyang 471000,Henan,China)

机构地区：[1]河南科技大学农学院,河南洛阳471000 [2]武昌理工学院生命科学学院,湖北武汉430223 [3]洛阳市农林科学研究院/中国农业科学院洛阳旱地农业试验基地,河南洛阳471000

出　　处：《作物学报》2025年第3期632-649,I0688-I0697,共28页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(32372227);河南省高等学校青年骨干教师培养计划(2021GGJS050);河南省科技研发计划联合基金项目(232103810021)资助。

摘　　要：蔗糖玉米非发酵-1相关蛋白激酶(Sucrose non-ferment-1-related protein kinase,SnRK)在响应非生物胁迫过程中发挥着核心调控作用。为系统分析小麦(Triticum aestivum L.)TaSnRK基因家族成员的基本理化性质、染色体分布、基因结构、系统进化关系和在局部根区干旱下的表达特性,本研究利用生物信息学的方法在小麦全基因组进行鉴定,并通过小麦公共表达数据库和实时荧光定量PCR(Real-time fluorescent quantitative PCR,qRT-PCR)分析了其在局部根区干旱下的表达模式。结果表明,在小麦中共鉴定到139个SnRK基因家族成员,并将其分为3个亚家族,每个亚家族中分别含有15(SnRK1)、31(SnRK2)和93(SnRK3)个成员,蛋白质序列长度在154~836个氨基酸之间。通过保守基序分析发现,3个亚家族的成员中均含Motif2和Motif4;在SnRK1亚家族中所有成员均含Motif14和Motif15,而在SnRK2和SnRK3亚家族中均不含这2个结构域;在SnRK3亚家族中所有成员均含Motif10,而在SnRK1和SnRK2亚家族中均不含Motif10。通过种内共线性分析发现,TaSnRK基因共有217个重复事件,同源性较高且进化过程非常保守,K_(a)/K_(s)比率显示仅有4对家族成员受到了正向的自然选择压力。顺式作用元件分析发现,小麦TaSnRK基因中的顺式作用元件大多与生长发育有关,此外还包含多种逆境响应的结合元件。基因表达模式分析显示,在TaSnRK家族成员中仅有20个基因在籽粒中的相对表达量较高,而在穗、叶、芽、根中分别有85、90、92和80个基因具有较高的表达量。qRT-PCR分析表明,TaSnRK基因在抗旱性强的小麦中表达量更高,另外SnRK2和SnRK3这2个亚族中的成员可以感受并传递干旱胁迫信号。蛋白互作分析结果表明,35个TaSnRK蛋白和与其相关的23个功能蛋白共存在267对蛋白互作事件。上述结果为深入研究TaSnRK基因在调控小麦生长发育与干旱胁迫中的响应提供了理论依据。Sucrose non-fermenting-1-related protein kinase(SnRK)plays a critical regulatory role in response to abiotic stress.To systematically analyze the physicochemical properties,chromosome distribution,gene structure,phylogenetic relationships,and expression characteristics of the TaSnRK gene family in wheat(Triticum aestivum L.)under local root zone drought stress,this study employed bioinformatics approaches to identify the full complement of TaSnRK genes in the wheat genome.Their expression patterns under local root zone drought stress were analyzed using a wheat expression database and quantitative real-time PCR(qRT-PCR).The analysis identified 139 members of the SnRK gene family in wheat,which were categorized into three subfamilies:15 members in SnRK1,31 in SnRK2,and 93 in SnRK3.Protein sequence lengths ranged from 154 to 836 amino acids.Conserved motif analysis revealed that all members of the three subfamilies shared Motif2 and Motif4.Additionally,all SnRK1 members contained Motif14 and Motif15,which were absent in SnRK2 and SnRK3 subfamilies.In contrast,all SnRK3 members contained Motif10,which was not found in SnRK1 and SnRK2 subfamilies.Intraspecific collinearity analysis indicated that the TaSnRK genes had a total of 217 duplication events,showing high homology and strong conservation during evolution.The K_(a)/K_(s) ratio suggested that only four pairs of TaSnRK genes were under positive selection pressure.Cis-regulatory element analysis revealed that most of the cis-elements in the TaSnRK genes were associated with growth and development,as well as various stress-responsive elements.Gene expression pattern analysis showed that only 20 TaSnRK genes exhibited relatively high expression levels in grains,whereas 85,90,92,and 80 genes were highly expressed in panicles,leaves,buds,and roots,respectively.qRT-PCR analysis confirmed that TaSnRK expression was higher in drought-resistant wheat varieties,with SnRK2 and SnRK3 subfamily members playing key roles in sensing and transmitting drought stress signals.Prot

关 键 词：小麦 TaSnRK基因 生物信息学 局部根区干旱 基因表达 

分 类 号：S512.1[农业科学—作物学]