苏丹草种子萌发期耐盐性评价及耐盐种质筛选  

Evaluation of salt tolerance and screening of salt-tolerant germplasm of Sorghum sudanese during seed germination period

作　　者：蒋优 马雪融 张博 李陈建[1,2] JIANG You;MA Xue-Rong;ZHANG Bo;LI Chen-Jian(Laboratory of Grassland Resources and Ecology in Western Arid Desert Region,Urumqi 830052,Xinjiang,China;College of Grassland Science,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China)

机构地区：[1]西部干旱荒漠区草地资源与生态实验室,新疆乌鲁木齐830052 [2]新疆农业大学草业学院,新疆乌鲁木齐830052

出　　处：《作物学报》2025年第3期835-844,共10页Acta Agronomica Sinica

基　　金：新疆维吾尔自治区自然科学基金项目(2022D01A72)资助。

摘　　要：盐碱地在我国分布较为广泛,选育耐盐牧草种质资源是开发利用盐碱地的重要手段。本研究以94份苏丹草种质资源为材料,通过室内模拟盐胁迫环境,于萌发期测定发芽势、发芽率、根长、芽长等11项指标,并对各个测量指标的相对值进行相关性分析、主成分分析和聚类分析,利用隶属函数法对苏丹草耐盐性进行综合评价。结果表明,盐胁迫下各指标存在广泛变异,经过主成分分析后,11项指标降维至3个综合指标,累计方差贡献率高达85.807%;通过聚类分析将94份种质资源分为五大类,最终筛选出高耐种质包括NX035、GS0218、SCH03-128、GS0227、GS479、GS477、283和GS1289,高敏种质00276、B478;并构建了逐步回归模型D=0.402RGI+0.397RRDW+0.261RGR-0.01(R^(2)=0.949,P<0.01),在200 mmol L^(-1)Na Cl盐胁迫下,相对发芽指数、相对根干重及相对发芽率可以作为苏丹草萌发期耐盐性评价关键指标。这些成果为未来苏丹草耐盐性育种提供了重要的材料基础。Saline-alkali soil is widely distributed across China,and breeding salt-tolerant forage grass germplasm is crucial for the development and utilization of such soils.In this experiment,94 genetic resources of Sorghum sudanense were subjected to salt stress in a controlled indoor environment.During the germination phase,various parameters such as seed viability,growth rate,root elongation,and shoot development were carefully assessed.Correlation analysis,principal component analysis,and cluster analysis were conducted on the relative values of each indicator,and the membership function method was applied for a comprehensive evaluation of salt tolerance in Sorghum sudanense.The results revealed significant variation in the measured indicators under salt stress.After principal component analysis,the 11 indicators were reduced to three composite indicators,with a cumulative variance contribution rate of 85.807%.Cluster analysis grouped the 94 germplasm resources into five major clusters.Among these,highly salt-tolerant germplasm included NX035,GS0218,SCH03-128,GS0227,GS479,GS477,283,and GS1289,while highly sensitive germplasm included 00276 and B478.A stepwise regression model,D=0.402RGI+0.397RRDW+0.261RGR-0.01(R^(2)=0.949,P<0.01)was constructed,indicating that the relative germination index,relative root dry weight,and relative germination rate can serve as key indicators for evaluating salt tolerance in Sorghum sudanense during the germination stage under 200 mmol L^(-1) NaCl salt stress.These findings provide a valuable material base for future breeding of Sorghum sudanense with enhanced salt tolerance.

关 键 词：苏丹草 萌发期 耐盐性评价 聚类分析 

分 类 号：S544.1[农业科学—作物学]