基于Fisher判别分析的槐花、炒槐花标准汤剂特征图谱差异性研究  

Study on the Differences of the Characteristic Mapping of Standard Decoction of Huaihua and Fried Huaihua Based on Fisher Discriminant Analysis

作　　者：高晗 孙福仁 李争 李军山 梁彩娟 廖明丽 李振江 GAO Han;SUN Furen;LI Zheng;LI Junshan;LIANG Caijuan;LIAO Mingli;LI Zhenjiang(China Shineway Pharmaceutical Group Ltd.,Shijiazhuang 051430,China;Shijiazhuang Industrial Technology Research Institute of Traditional Chinese Medicine Formula Granules,Shijiazhuang 050002,China;Yunnan Shineway Spirin Pharmaceutical Co.,Chuxiong 675000,China)

机构地区：[1]神威药业集团有限公司,河北石家庄051430 [2]石家庄市中药配方颗粒产业技术研究院,河北石家庄050002 [3]云南神威施普瑞药业有限公司,云南楚雄675000

出　　处：《西部中医药》2025年第1期11-17,共7页Western Journal of Traditional Chinese Medicine

基　　金：云南省重大科技专项计划(202102AA310027);云南省配方颗粒重点实验室项目(202105AG070014);石家庄市中药配方颗粒产业技术研究院项目(228790779A)。

摘　　要：目的:建立槐花、炒槐花标准汤剂特征图谱,区分图谱差异。方法:采用了UPLC色谱仪与WATERS BEH C18(2.1mm×100 mm,1.7μm)色谱柱,以乙腈-0.1%磷酸水为流动相,柱温为35℃,流速为0.3 mL/min,检测波长为257 nm。结果:应用特征图谱提取槐花、炒槐花标准汤剂特征图谱中特征成分,共确定5个特征峰。结论:槐花、炒槐花标准汤剂UPLC特征图谱方法可靠、稳定,可用于槐花、炒槐花配方颗粒的定性鉴别。Objective:To establish the feature chromatograms of standard decoction of Huaihua(Sophora japonica L)and fried one,and to distinguish the differences in the mapping.Methods:A UPLC chromatographic instru-ment and WATERS BEH C18(2.1 mm×100 mm,1.7μm)chromatographic column were employed,with acetonitrile-0.1%aqueous phosphate as the mobile phase,column temperature 35℃,flow rate 0.3 mL/min and the detection wavelength 257 nm.Results:Five characteristic peaks were confirmed through the extraction of characteristic components from standard decoction of Huaihua and fried Huaihua with characteristic mapping.Conclusion:The method of UPLC characteristic spectrum of Huaihua and fried Huaihua,which is reliable and stable,could be used for qualitative identification of the formula granules.

关 键 词：槐花 炒槐花 标准汤剂 特征图谱 芦丁 槲皮素 FISHER判别分析 

分 类 号：R284.1[医药卫生—中药学]