基于异病同治及数据挖掘探讨八珍汤治疗股骨头坏死及骨肉瘤的机制  

Potential Mechanism of Treating Osteonecrosis of the Femoral Head and Osteosarcoma with Bazhen Tang Based on Data Mining and Same Treatment for Different Diseases

作　　者：潘海达 秦刚[2] PAN Haida;QIN Gang(Yongjia County Hospital of Traditional Chinese Medicine,Yongjia 325100,China;The First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530023,China)

机构地区：[1]永嘉县中医医院,浙江永嘉325100 [2]广西中医药大学第一附属医院,广西南宁530023

出　　处：《西部中医药》2025年第1期88-94,共7页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81860793);广西自然科学基金(2020JJA140375)。

摘　　要：目的:基于网络药理学联合分析对接法探究八珍汤治疗股骨头坏死(osteonecrosis of the femoral head,ONFH)及骨肉瘤(osteosarcoma,OS)的潜在机制。方法:通过中药系统药理学数据库与分析平台(traditional Chinese medicine systems pharmacology database and analysis plsatform,TCMSP)下载活性成分和相关靶点,通过人类基因数据库(the human gene database,GeneCards)、在线人类孟德尔遗传数据库(online mendelian inheritance in man,OMIM)下载ONFH、OS相关疾病靶点。使用R软件取交集后,再与两病共有基因取交集。利用STRING数据库对交集靶点构建蛋白互作网络图,利用R软件进行基因本体(gene ontology,GO)与京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)富集分析,运行Autodock Vina对活性成分及关键蛋白进行对接。结果:筛选获得八珍汤疾病相关有效成分共159个,相关靶点1965个,ONFH、OS共有靶点615个,药物与两种疾病交集获得药物疾病靶点124个。GO富集分析获得BP相关条目2082个,CC相关条目42个,MF相关条目122个。KEGG功能富集分析发现糖尿病并发症中的高级糖基化终末产物-受体(advanced glycation end products-receptor,AGE-RAGE)、脂质与动脉粥样硬化(lipid and atherosclerosis)、乙型肝炎(hepatitis B)、前列腺癌(prostate cancer)、胰腺癌(pancreatic cancer)等161条信号通路,分子对接初步验证了八珍汤主要活性成分能与关键靶点发生相互作用。结论:Akt1作为关键基因,通过调节局部血管微循环、骨代谢达到治疗ONFH、OS的目的,为后续研究八珍汤治疗ONFH、OS的临床应用具有指导意义,并从现代医学的角度诠释中医中药异病同治的原理。Objective:To probe into the potential mechanism of Bazhen Tang in the treatment of osteonecrosis of the femoral head(ONFH)and osteosarcoma(OS)based on network pharmacology co-analysis docking method.Methods:The active ingredients and the relevant targets were downloaded from TCMSP,and the ONFH and OS related targets were downloaded from GeneCards and OMIM.R software was used for the intersection of both,and the intersection with the genes shared by the two diseases.STRING database was utilized to build protein-protein interactions(PPI),R software was applied to conduct GO and KEGG enrichment analysis,the docking of active ingredients and key protein was performed using Autodock Vina.Results:The study has yielded 159 active ingredients related to Bazhen Tang and disease,1965 related targets,615 shared targets between ONFH and OS,and 124 medicine-disease targets obtained from the intersection of medicine and two diseases.2082 BPrelated items,42 cell component(CC)related items and 122 molecular function(MF)related items were obtained from GO enrichment analysis.All 161 signaling pathways including AGE-RAGE in diabetic complications,hepatitis B,prostate cancer,pancreatic cancer and others were gained through KEGG functional enrichment analysis,molecular docking primarily revealed that the main active ingredients of Bazhen Tang could interact with key targets.Conclusion:Akt1 as the key gene could treat ONFH and OS by regulating local vascular microcirculation and bone metabolism,it has the significance of guidance for clinical application of the subsequent study on the treatment of ONFH and OS with Bazhen Tang,and expounds the principle of same treatment for different diseases in TCM from the angle of modern medicine.

关 键 词：股骨头坏死 骨肉瘤 网络药理学 八珍汤 异病同治 

分 类 号：R274.9[医药卫生—中西医结合]