转录组和代谢组联合分析茶树对茶饼病病原菌的防御反应  

Responses of Tea Plants to Blister Blight Analyzed Using Transcriptome and Metabolome

作　　者：姚玉仙 张明泽 舒烨 罗福丹 全昌彬 刘荣 向志攀 梅鑫 YAO Yuxian;ZHANG Mingze;SHU Ye;LUO Fudan;QUAN Changbin;LIU Rong;XIANG Zhipan;MEI Xin(College of Biological Science and Agriculture,Qiannan Normal University for Nationalities,Duyun,Guizhou 558000,China;Key Laboratory of Tea Research for Characteristic Components,Guizhou Provincial Higher Education Institutions,Duyun,Guizhou 558000,China)

机构地区：[1]黔南民族师范学院生物科学与农学院,贵州都匀558000 [2]贵州省高等学校茶树特征性成分研究重点实验室,贵州都匀558000

出　　处：《福建农业学报》2024年第11期1265-1279,共15页Fujian Journal of Agricultural Sciences

基　　金：贵州省基础研究(自然科学)项目(黔科合基础[2020]1Y120);贵州省教育厅自然科学基金项目(黔教合KY字[2019]211);贵州省教育厅贵州省高等学校茶树特征性成分研究重点实验室项目(黔教技[2023]027号);贵州省大学生创新创业训练计划项目(S202210670021)。

摘　　要：【目的】探明茶树对茶饼病菌的抗性分子机制,挖掘抗病相关基因,为茶树抗性育种提供依据。【方法】通过转录组测序和代谢组分析,比较茶树健康叶片(CK)和感染茶饼病的叶片(TB)中的差异表达基因(differentially expressed genes,DEGs)和差异代谢物(differentially accumulated metabolites,DAMs)。【结果】转录组数据显示,样品CK和TB之间共有1009个DEGs;GO富集分析表明,差异基因参与了细胞壁代谢及调控几丁质酶活性、氧化还原酶活性和木葡聚糖:木葡基转移酶活性;KEGG代谢途经分析表明,DEGs显著富集在“类黄酮生物合成”“苯丙素生物合成”“氨基糖和核苷糖代谢”“甘油酯代谢”和“二苯乙烯、二芳基庚烷和姜酚生物合成”途径;DEGs中包含47个转录因子,分属21个转录因子家族,主要包括bHLH、SBP、AP2/ERF-AP2和MYB等,这些转录因子可能是茶树抵御茶饼病侵染过程中重要的调控基因。利用广泛靶向代谢组学技术分析,共发现353个DAMs,DAMs主要富集于“类黄酮生物合成”“赖氨酸生物合成”和“丙氨酸、天门冬氨酸和谷氨酸代谢”途径。利用转录组联合代谢组分析发现,显著共同富集的途径是“类黄酮生物合成”“苯丙素生物合成”和“二苯乙烯、二芳基庚烷和姜酚生物合成”;筛选了与苯丙素类及类黄酮生物合成途径相关的20个DEGs和15个DAMs,其中,CSS0011741(4CL)、CSS0002940(DFR)、CSS0015968(DFR)和CSS0010687(ANS)等DEGs在感病叶中上调表达,根皮素、根皮苷、4-羟基苯乙烯、对香豆酰奎宁酸、二氢杨梅素、表没食子儿茶素和芍药素-3-O-葡萄糖苷等DAMs在感病叶中积累。【结论】“苯丙素类生物合成”和“类黄酮生物合成”等代谢途径中的DEGs在茶树响应茶饼病侵染中发挥重要作用,根皮素、根皮苷以及表没食子儿茶素等DAMs可能是茶树抵御茶饼病侵染的重要次生代谢产物。【Objective】Resistance mechanism and associated genes of tea plants in response to blister blight infection were studied based on transcriptome as well as metabolome.【Methods】Differentially expressed genes(DEGs)and differentially accumulated metabolites(DAMs)in leaves of healthy(CK)and blister blight-infected(TB)tea plants were compared by using both transcriptome and metabolome.【Results】Between CK and TB,1009 DEGs associated with the cell wall metabolism and the regulations of chitinase,oxidoreductase,and xyloglucan:xyloglucanosyltransferase activities were identified by a GO enrichment analysis.The KEGG analysis showed the DEGs significantly enriched in the pathways of flavonoid biosynthesis,phenylpropanoid biosynthesis,amino sugar and nucleotide sugar metabolism,glycerolipid metabolism,and stilbenoid,diarylheptanoid,and gingerol biosynthesis.Forty-seven transcription factors in the DEGs,which belonged to 21 transcription factor families including bHLH,SBP,AP2/ERF-AP2,and MYB,might be the genes significantly involved in the resistance of a tea plant to blister blight.The 353 DAMs identified by using the widely targeted metabolomics were enriched mainly in the flavonoid biosynthesis and lysine biosynthesis as well as the alanine,aspartate,and glutamate metabolism pathways.The transcriptome and metabolome revealed significant co-enrichments in the flavonoid biosynthesis,phenylpropanoid biosynthesis,and stilbenoid,diarylheptanoid,and gingerol biosynthesis pathways.Among the selected 20 DEGs and 15 DAMs associated with the phenylpropanoids and flavonoids biosynthesis pathways,DEGs such as CSS0011741(4CL),CSS0002940(DFR),CSS0015968(DFR),and CSS0010687(ANS)were upregulated,while DAMs such as phloretin,phlorizin,4-Hydroxystyrene,p-Coumaroyl quinic acid,dihydromyricetin,epigallocatechin,and peonidin 3-O-glucoside were accumulated in the infected leaves.【Conclusion】The DEGs in the phenylpropanoid and flavonoid biosynthesis pathways might play a crucial role in the response of tea plants to blister blight.

关 键 词：茶饼病 转录组 代谢组 差异表达基因 差异代谢物 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]