基于血清TLR4/NF-κB信号通路探讨中风回春片联合复方丹参注射液在急性脑梗死治疗中的作用机制研究  

The Mechanism of ZhongfengHuichun Tablets combined with Compound Danshen Injection in the Treatment of Acute Cerebral Infarction was Studied Based on the Serum TLR4/NF-κB Signaling Pathway

作　　者：李学慧 王建峰 LI Xuehui;WANG Jianfeng(Zhengzhou 460 hospital,Zhengzhou450000,Henan,China)

机构地区：[1]郑州四六0医院,河南郑州450000

出　　处：《实用中医内科杂志》2025年第2期138-142,共5页Journal of Practical Traditional Chinese Internal Medicine

基　　金：郑州市科技攻关计划项目(20220076)。

摘　　要：目的研究中风回春片与复方丹参注射液结合治疗对老年急性脑梗死患者脑部血液流动及TLR4/NF-κB信号通路在血清中的影响。方法以我院就诊的150例急性脑梗死患者为研究对象,随机均分为三组(联合组、对照A、B组)。均给予常规治疗,对照A组加用中风回春片,对照B组加用复方丹参注射液,而联合组则同时应用这两种药物,疗程均为2周。疗程结束后,对三组患者的治疗效果与不良反应进行了比较,同时观察了NIHSS、MMSE及BI评分在治疗前后的变化。此外,还评估了脑血流动力学参数,包括平均血流量(Qmean)、血流速度(Vmean)、脑血管特征阻抗(ZCV)及外周阻力(R),并检测了血清中GFAP、S100B、NSE等神经损伤标志物的水平,进一步分析了TLR4/NF-κB信号通路的表达状态。结果与对照A、B两组比较,联合治疗组的总有效率显著提高(P<0.05),且在不良反应方面,三组之间未观察到明显差异(P>0.05)。为期两周的治疗后,联合组在NIHSS、MMSE及BI评分的提升上均明显优于两对照组(P<0.05)。此外,三组的Qmean和Vmean水平均有所提高,而ZCV、R水平以及血清中的GFAP、S100B、NSE水平均有所下降,其中联合组的改善最为明显(P<0.05)。在治疗两周后,三组血清中的TLR4和NF-κBP65蛋白表达量均有所降低,联合组的降低幅度显著大于两个对照组(P<0.05)。结论中风回春片与复方丹参注射液的联合疗法不仅能提升老年急性脑梗死患者的疗效,同时确保了治疗的安全性。该治疗方法能优化脑部血流,调节TLR4/NF-κB信号传导,缓解神经损害,进而有效促进患者神经认知功能与日常自理能力的恢复。Objective To investigate the effect of ZhongfengHuichun Tablets combined with Compound Danshen Injection on cerebral blood flow and TLR4/NF-κB signaling pathway in serum of elderly patients with acute cerebral infarction.Methods 150 patients with acute cerebral infarction treated in our hospital were randomly divided into three groups(combined group,control group A and B).All patients received routine treatment.The control group A was treated with ZhongfengHuichun Tablets,the control group B with Compound Danshen Injection,and the combined group with both drugs simultaneously.The course of treatment was 2 weeks.After the course of treatment,therapeutic effects and side effects of the three groups were compared,and the changes of NIHSS,MMSE and BI scores before and after treatment were observed.In addition,cerebral hemodynamic parameters,including mean blood flow(Qmean),blood flow velocity(Vmean),cerebrovascular characteristic impedance(ZCV)and peripheral resistance(R),were evaluated.The levels of nerve injury markers such as GFAP,S100 B and NSE in serum were detected,and the expression of TLR4/NF-κB signaling pathway was further analyzed.Results Compared with the control group A and B,the total effective rate of the combined treatment group was significantly improved(P<0.05).After two weeks of treatment,the improvement of NIHSS,MMSE and BI scores in the combined group was significantly better than that in the two control groups(P<0.05).In addition,the levels of Qmean and Vmean in the three groups were increased,while the levels of ZCV,R and the levels of GFAP,S100 B and NSE in serum were decreased,and the improvement of the combined group was the most obvious(P<0.05).After two weeks of treatment,the expression of TLR4 and NF-κBP65 protein in the serum of the three groups decreased,and the decrease in the combined group was significantly greater than that in the two control groups(P<0.05).Conclusion The combined therapy of ZhongfengHuichun Tablets and Compound Danshen Injection can not only improve the efficacy

关 键 词：急性脑梗死 中风回春片 复方丹参注射液 神经损伤因子 Toll样受体4/核因子-κB信号通路 

分 类 号：R277.7[医药卫生—中医学]