基于硅晶负载银纳米基底的唾液中罂粟的检测  

Identification and classification of opium in oral fluid based on Ag nanoassemblies on Si surface

作　　者：崔胜峰 孙国良 田雨欣 万敬伟 陈娟 周成合 CUI Sheng-feng;SUN Guo-liang;TIAN Yu-xin;WAN Jin-wei;CHEN Juan;ZHOU Cheng-he(Center for Traffic Evidence Technology,Department of Criminal Science and Technology,Zhengzhou Police College,Zhengzhou 450053,China;Institute of Public Safety,Zhengzhou University,Zhengzhou 450001,China;Key Laboratory of Applied Chemistry of Chongqing Municipality,School of Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)

机构地区：[1]郑州警察学院刑事科学技术系,交通物证技术研究中心,河南郑州450053 [2]郑州大学公共安全研究院,河南郑州450001 [3]西南大学化学化工学院,重庆市高校应用化学重点实验室,重庆400715

出　　处：《化学研究与应用》2025年第2期302-309,共8页Chemical Research and Application

基　　金：国家自然科学基金青年基金项目(201805208)资助;河南省科技攻关项目(212102310487)资助。

摘　　要：铁路站车大客流场景下吸毒人员唾液中罂粟毒品的快速检验成为近年来化学和法庭科学研究的重点和热点之一。本文通过一步氧化还原反应在硅晶圆表面负载上具有高密度纳米尖端和深纳米间隙的三维银纳米颗粒层,高效地制备得到表面增强拉曼散射(SERS)的银纳米基底。研究结果发现在含有罂粟的唾液复杂基质中,通过调节体系酸碱度,新制备的银纳米基底以静电相互作用的方式吸附质子化的罂粟活性分子,从而产生表面等离子共振,最终获得稳定、高质量的表面增强拉曼光谱。并且银纳米基底对吗啡分子具有高度的选择性,可以在混合唾液基质中快速、准确地对吗啡分子进行选择性吸附,检测浓度低至纳克级别。In recent years,the rapid detection of opium in oral fluid has become one of the research focuses in the field of chemistry and forensic science in large passenger flow scenarios at railway stations.In this work,SERS silver nanosubstrates were obtained by efficiently loading silver nanoparticles with high density nanotips and deep nanogaps on the surface of silicon wafers by a one-step redox reaction.The research findings indicate that within the intricate matrix of oral fluid containing opium,by adjusting the pH of the system,the newly prepared Ag nanosubstrate can adsorb protonated opium's active molecules through electrostatic interactions,thereby generating surface plasmon resonance,and ultimately achieving stable,high-quality surface-enhanced Raman spectroscopy signals.Moreover,the newly fabricated Ag nanosubstrate exhibits high selectivity for morphine molecules,enabling rapid and accurate selective adsorption of morphine in the complex saliva matrix,with a detection sensitivity that reaches down to the nanogram level.

关 键 词：吗啡 罂粟 SERS 银纳米 

分 类 号：O657.3[理学—分析化学]