荧光探针的构建及其对黄曲霉素B1的检测  

Construction of fluorescent probes and the detection of aflatoxin B1

作　　者：麦子悦 张晓辉 钟佳鹏 何怡琳 李妙灵 翟海云 MAI Zi-yue;ZHANG Xiao-hui;ZHONG Jia-peng;HE Yi-lin;LI Miao-lin;ZHAI Hai-yun(Guangdong Pharmaceutical University,College of Pharmacy,Guangzhou 51006,China)

机构地区：[1]广东药科大学药学院,广东广州510006

出　　处：《化学研究与应用》2025年第2期319-326,共8页Chemical Research and Application

基　　金：广东省大学生创新创业训练项目(202310573063)资助。

摘　　要：通过简便的水热方法制备了Eu3+功能化的DUT-52型MOF(Eu/DUT-52),并将其用于黄曲霉素B1(AFB1)的荧光检测。Eu/DUT-52利用Eu3+的特征发射作为内部校正的参比信号,同时利用DUT-52的荧光强度随AFB1浓度增加而增加的特点,实现AFB1的灵敏、准确和可视化的检测。本文对Eu/DUT-52的结构、发光性能、检测性能进行了详细的表征和分析,AFB1的线性范围为0~1μg·mL^(-1),检出限为2 ng·mL^(-1),并在实际样品薏米中验证了其检测效果,回收率为93.50%~99.74%。Eu~(3+)-functionalized DUT-52 MOF(Eu/DUT-52)was prepared through a simple hydrothermal method and applied for the fluorescence detection of aflatoxin B1(AFB1).Eu/DUT-52 utilizes the characteristic emission of Eu~(3+)as an internal reference signal for calibration.Simultaneously,it takes advantage of the feature that the fluorescence intensity of DUT-52 increases with the concentration of AFB1,resulting in sensitive,accurate and visual detection of AFB1.This study provides a detailed characterization and analysis of the structure,luminescent properties,and detection performance of Eu/DUT-52.The linear range for AFB1 detection is0~1μg·mL^(-1),with a detection limit of 2 ng·mL^(-1).The effectiveness of the detection was validated in actual samples of coix seed sample,showing recovery yields ranging from 93.50%to 99.74%.

关 键 词：黄曲霉素B1 荧光法 比率型荧光 金属有机骨架 

分 类 号：O657.3[理学—分析化学]