短期氮添加下不同树种土壤可溶性有机质的变化及其与真菌群落的关联  

Change of soil dissolved organic matter and its association with fungal communities after short-term nitrogen addition in different tree species

作　　者：柏欣宇 元晓春 曾泉鑫 张晓晴 孙浩 张秋芳[1,2] 卢姣宏[1,2] 崔琚琰 陈岳民 BAI Xinyu;YUAN Xiaochun;ZENG Quanxin;ZHANG Xiaoqing;SUN Hao;ZHANG Qiufang;LU Jiaohong;CUI Juyan;CHEN Yuemin(School of Geographical Science,Fujian Normal University,Fuzhou 350007,China;Cultivation Base of State Key Laboratory of Subtropical Mountain Ecology,Fujian Normal University,Fuzhou 350007,China;College of Tourism,Wuyi University,Wuyishan 354300,Fujian,China;School of Resources and Environmental Engineering,Anhui University,Hefei 230601,China;School of Architectural Engineering,Tongling College,Tongling 244061,Anhui,China)

机构地区：[1]福建师范大学地理科学学院,福州350007 [2]福建师范大学湿润亚热带山地生态国家重点实验室培育基地,福州350007 [3]武夷学院旅游学院,福建武夷山354300 [4]安徽大学资源与环境工程学院,合肥230601 [5]铜陵学院建筑工程学院,安徽铜陵244061

出　　处：《应用生态学报》2025年第1期67-76,共10页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目(32201532,32371846);安徽省高校自然科学研究项目(2023AH051667)资助。

摘　　要：土壤可溶性有机质(DOM)是土壤碳库的重要组成部分,其与土壤真菌群落的相互作用对土壤碳循环至关重要。本研究在黄山松和罗浮栲林中分别设置3个氮添加水平,包括对照(0 kg N·hm^(-2)·a^(-1))、低氮(40 kg N·hm^(-2)·a^(-1))、高氮(80 kg N·hm^(-2)·a^(-1)),并采用三维荧光与平行因子分析联用,结合高通量测序,探讨了氮添加对土壤DOM的数量和光学特性的影响及其与真菌群落的关联。结果表明:在黄山松林中,氮添加下土壤DOM数量和光学特性以及土壤真菌Chao1指数均无显著变化;而在罗浮栲林中,氮添加下土壤可溶性有机碳含量、DOM的腐殖化指数和类腐殖质组分的相对含量降低,尤其在高氮处理下,土壤真菌群落中被孢霉门的相对丰度显著增加。冗余分析结果显示,黄山松林土壤DOM数量和光学特性主要受土壤真菌Chao1指数的影响,而罗浮栲林土壤DOM数量和光学特性的主要影响因子为真菌群落结构、β-葡糖苷酶活性和土壤pH。这说明短期氮添加下不同树种土壤DOM数量和光学特性的主要影响因子不同。网络分析发现,土壤真菌群落与DOM组分之间存在密切联系,而氮添加下土壤真菌群落与DOM组分的网络关联边减少,说明两者的相互作用被削弱。本研究可为氮沉降下森林土壤真菌群落与土壤可溶性碳库之间的联系提供新的见解。Soil dissolved organic matter(DOM) is an important component of soil carbon pool, and its interaction with soil fungal communities is crucial for soil carbon cycle. We set up three nitrogen(N) addition levels, including control(0 kg N·hm^(-2)·a^(-1)), low N(40 kg N·hm^(-2)·a^(-1)), and high N(80 kg N·hm^(-2)·a^(-1)), in Pinus taiwanensis and Castanopsis faberi forests to explore the effect of N addition on the DOM quantity and optical properties, as well as the relationship between soil fungal communities and DOM by using three-dimensional fluorescence spectroscopy coupled with parallel factor analysis and high-throughput sequencing. The results showed that N addition did not significantly change the quantity or optical properties of soil DOM and Chao1 index in P. taiwanensis forest. In contrast, N addition, especially the high addition rate, significantly reduced the content of soil dissolved organic carbon, humification index, and relative content of humic-like components of DOM in C. faberi forest. Additionally, the relative abundance of Mortierellomycota in the fungal community significantly increased after N addition. Redundancy analysis indicated that soil DOM content and optical properties in P. taiwanensis forest were mainly influenced by Chao1 index after N addition. In contrast, soil fungal community structure, β-glucosidase activity, and pH were the main factors influencing DOM content and optical properties in C. faberi forests. This suggested that the key factors affecting soil DOM quantity and optical properties after short-term N addition depended on tree species. Furthermore, network analysis demonstrated a close relationship between soil fungal communities and DOM components. Fewer associative edges were observed, suggesting that the association between soil fungal communities and DOM components was weakened by N addition. Overall, this study provided novel insights into the relationship between fungal communities and dissolved organic carbon pools in forest soils with N addition.

关 键 词：氮沉降 树种 可溶性有机质 真菌群落 共现网络 

分 类 号：S714[农业科学—林学]