PCOS患者血清miR-93-5p和miR-98-3p的表达情况及早期诊断和预后价值  

Expression of serum miR-93-5p and miR-98-3p and their early diagnostic and prognostic value in PCOS patients

作　　者：柯妙芳 谢玲玲 林碧蓉 KE Miao-fang;XIE Ling-ling;LIN Bi-rong(Obstetrics and Gynecology Department,the Second Hospital of Zhangzhou,Zhangzhou 363199)

机构地区：[1]漳州市第二医院妇产科,漳州363199

出　　处：《生殖医学杂志》2025年第2期179-185,共7页Journal of Reproductive Medicine

摘　　要：目的探讨多囊卵巢综合征(PCOS)患者血清微小RNA-93-5p(miR-93-5p)和微小RNA-98-3p(miR-98-3p)的表达情况及其对早期诊断和预后的价值。方法选取2019年12月至2023年6月于本院就诊治疗的104例PCOS患者为研究对象(PCOS组),选择同期于本院进行体检的104例健康女性作为对照组,并根据预后情况将PCOS组分为预后不良组(n=42)和预后良好组(n=62)。采用RT-qPCR法检测血清miR-93-5p、miR-98-3p水平;采用多因素Logistic回归分析PCOS患者预后的影响因素;采用受试者工作特征(ROC)曲线分析血清miR-93-5p、miR-98-3p对PCOS早期诊断及预后预测的价值。结果PCOS组体质量指数(BMI)、高密度脂蛋白胆固醇(HDL-C)、卵巢髓质面积、卵巢总面积、E2、LH水平及miR-93-5p、miR-98-3p水平均显著高于对照组,FSH水平显著低于对照组(P均<0.05);PCOS预后不良组的BMI、LH、miR-93-5p、miR-98-3p水平均显著高于预后良好组(P<0.05)。Logistic回归分析显示,miR-93-5p[OR=2.781,95%CI(1.211,6.384),P=0.016]、miR-98-3p[OR=2.175,95%CI(1.166,4.056),P=0.015]是PCOS患者预后不良的影响因素。血清miR-93-5p、miR-98-3p联合诊断PCOS的曲线下面积(AUC)为0.833,敏感性和特异性分别为60.58%和92.31%,显著优于miR-93-5p(Z=3.395,P=0.007)、miR-98-3p(Z=2.200,P=0.027)单独诊断;血清miR-93-5p、miR-98-3p二者联合预测PCOS患者预后的AUC为0.952,敏感性和特异性分别为95.24%和83.87%,显著优于miR-93-5p(Z=2.687,P=0.007)、miR-98-3p(Z=2.340,P=0.019)单独预测。结论PCOS患者血清miR-93-5p、miR-98-3p水平明显上升,与患者预后有密切联系;二者联合对PCOS患者的早期诊断和预后预测具有较高价值。Objective:To explore the expression levels of serum microRNA-93-5p(miR-93-5p)and microRNA-98-3p(miR-98-3p)and their clinical significance for early diagnosis and prognosis in patients with PCOS.Methods:A total of 104 patients with PCOS who were admitted to the Second Hospital of Zhangzhou from December 2019 to June 2023 were recruited as the study subjects(PCOS group),while 104 healthy individuals who underwent medical examinations in the same hospital were collected as the control group.Based on the prognosis,patients in PCOS group were further divided into poor prognosis group(n=42)and good prognosis group(n=62).RT-qPCR method was applied to detect serum levels of miR-93-5p and miR-98-3p.Multivariate logistic regression was applied to analyze the factors influencing the prognosis of PCOS patients.Receiver operating characteristic(ROC)curve was applied to analyze the predictive value of serum miR-93-5p and miR-98-3p in early diagnosis and prognosis of PCOS.Results:The body mass index(BMI),high-density lipoprotein cholesterol(HDL-C),ovarian medullary area,total ovarian area,E 2,LH levels,and miR-93-5p,miR-98-3p levels in the PCOS group were significantly higher than those in the control group,while FSH levels were significantly lower than those in the control group(all P<0.05).The BMI,LH,serum levels of miR-93-5p and miR-98-3p in poor prognosis group were significantly higher than those in good prognosis group(P<0.05).Logistic regression analysis results showed that miR-93-5p[OR=2.781,95%CI(1.211,6.384),P=0.016]and miR-98-3p[OR=2.175,95%CI(1.166,4.056),P=0.015]were factors that affected the poor prognosis of PCOS patients.The area under the ROC curve(AUC)of serum miR-93-5p combined with miR-98-3p in the diagnosis of PCOS was 0.833,with sensitivity and specificity of 60.58%and 92.31%,respectively,which was significantly better than independent diagnoses of miR-93-5p(Z=3.395,P=0.007)and miR-98-3p(Z=2.200,P=0.027).The AUC of serum miR-93-5p combined with miR-98-3p in predicting the prognosis of PCOS patients was 0.9

关 键 词：多囊卵巢综合征 微小RNA-93-5p 微小RNA-98-3p 诊断 预后 

分 类 号：R711.75[医药卫生—妇产科学]