姜黄素对斑马鱼卵巢衰老的影响  

The Effects of Curcumin on The Ovarian Aging in Zebrafish

作　　者：陈伟庭 祝浈浈 杨燕君 彭嘉乐 莫桃 黄雅枝 赵祎帆 范玉琴[1] CHEN Weiting;ZHU Zhenzhen;YANG Yanjun;PENG Jiale;MO Tao;HUANG Yazhi;ZHAO Yifan;FAN Yuqin(School of Life Sciences/Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Area,Jiaying University,Meizhou 514015,China)

机构地区：[1]嘉应学院生命科学学院/广东省山区特色农业资源保护与精准利用重点实验室,梅州514015

出　　处：《华南师范大学学报(自然科学版)》2024年第6期121-128,共8页Journal of South China Normal University(Natural Science Edition)

基　　金：广东省自然科学基金项目(2018A030307041);广东省科技创新战略专项资金项目(2020329105820A000002);广东省教育厅重点科研平台建设项目(2021ZDJS072);嘉应学院人才科研启动项目(2022RC38);嘉应学院科研项目(2023KJY9)。

摘　　要：姜黄素具有抗氧化、抗炎等功能,能在一定程度上延缓机体的衰老进程。为进一步探究姜黄素在卵巢衰老方面的作用,利用斑马鱼为卵巢衰老模型,采用喂食的药物处理方法,对其持续处理1个月。药物处理的同时开展产卵功能的检测,直至药物处理结束。此外,对卵巢的组织学变化进行分析,并检测了生殖轴中相关基因的表达。实验结果显示:(1)4 mM和8 mM浓度的姜黄素处理,可在一定程度上提高衰老卵巢的产卵数量;(2)8 mM药物处理组的卵母细胞的受精率和受精卵的存活率得到较为明显的提高;(3)卵巢组织学检测表明8 mM药物处理组的卵巢处于较为健康的状态;(4)8 mM的姜黄素处理提高了gnrh3的表达,同时抑制了gh的表达;(5)卵巢中的arom基因的表达在4 mM和8 mM的药物处理组中都呈现下调趋势。本研究结果提示姜黄素可能通过调节生殖轴中的相关基因表达,改善了卵巢的健康状态,在一定程度上延缓了卵巢的衰老。Curcumin has antioxidant and anti-inflammatory properties that can help delay the aging process of the body to a certain extent.To further explore the role of curcumin in ovarian aging,zebrafish were used as an ovarian aging model,and the feeding drug treatment method was used for continuous treatment for 1 month.The spawning function was tested at the same time as the drug treatment until the end of the drug treatment.In addition,the histological changes of the ovaries were analyzed,and the expression of related genes in the reproductive axis was detected.The experimental results showed that:(1)Curcumin treatment at concentrations of 4 mM and 8 mM could increase the number of eggs laid by aging ovaries to a certain extent;(2)The fertilization rate of oocytes and the survival rate of fertilized eggs in the 8 mM drug treatment group were significantly improved;(3)Ovarian histological examination also showed that the ovaries in the 8 mM drug treatment group were in a healthier state;(4)8 mM curcumin treatment increased the expression of gnrh3 and inhibited the expression of gh;(5)The expression of arom in the ovaries showed a downward trend in both the 4 mM and 8 mM drug treatment groups.The results of this study suggest that curcumin may improve the health of the ovaries and delay the aging of the ovaries to a certain extent by regulating the expression of related genes in the reproductive axis.

关 键 词：姜黄素 斑马鱼 生殖衰老 

分 类 号：Q955[生物学—动物学]