长白山牛皮杜鹃ERM真菌Hyaloscypha hepaticicola及其生物学特性  

ERM fungus Hyaloscypha hepaticicola on Rhododendron aureum in Changbai Mountains and its biological characteristics

作　　者：苏丹 许榕凡 孙晓东 戴冠华[3] 吕国忠 杨红 SU Dan;XU Rongfan;SUN Xiaodong;DAI Guanhua;LYU Guozhong;YANG Hong(College of Life Science and Engineering,Shenyang University,Shenyang 110044,Liaoning,China;College of Environment and Resources,Dalian Minzu University,Dalian 116600,Liaoning,China;Changbai Mountain Research Station of Forest Ecosystem,Shenyang Institute of Applied Ecology,Chinese Academy of Sciences,Baihe 133613,Jilin,China)

机构地区：[1]沈阳大学生命科学与工程学院,辽宁沈阳110044 [2]大连民族大学环境与资源学院,辽宁大连116600 [3]中国科学院沈阳应用生态研究所长白山森林生态系统定位站,吉林白河133613

出　　处：《菌物学报》2025年第1期52-60,共9页Mycosystema

基　　金：国家自然科学基金(31170020,31770025);中央高校基本科研业务费。

摘　　要：牛皮杜鹃Rhododendron aureum是长白山自然保护区苔原带优势植被之一,为适应高山苔原带的贫瘠土壤和寒冷气候条件,其与部分土壤真菌形成的欧石南菌根ericoid mycorrhiza(ERM)为其生长和繁殖发挥了关键生态功能。本文以分离自长白山苔原带牛皮杜鹃根系的2株常见的ERM真菌为研究对象,通过形态学观察和ITS基因序列同源性比对及其系统进化分析确定其分类地位,并利用单因素试验研究其生物学特性。结果表明,通过形态学和rDNA ITS序列分析,2个菌株均鉴定为苔生晶杯菌Hyaloscypha hepaticicola。该菌最适培养条件为:葡萄糖为碳源,酵母粉为氮源,温度为20℃,pH 4,全黑暗培养。该菌适宜在相对低温和偏酸环境生长,与其在长白山苔原带生长的土壤条件和气候条件存在紧密联系。本研究为苔生晶杯菌及其他ERM真菌的深入研究和资源利用奠定了理论基础。Rhododendron aureum is one of the dominant plants in the tundra belt of Changbai Mountain Nature Reserve.In order to adapt itself to the environmental conditions of infertile soil and cold climate in the tundra of the mountains,R.aureum evolves a special ericoid mycorrhiza(ERM)with some soil fungi,which plays a key ecological role in the plant survival and propagation.Two isolates of ERM fungi were screened from the roots of R.aureum in the tundra of Changbai Mountains,and they were identified as Hyaloscypha hepaticicola by morphological observation and ITS sequence analysis.The study of the biological characteristics showed that the optimum culture conditions for this fungus were as follows:using glucose as carbon source and yeast powder as nitrogen source;cultivation at temperature of 20℃and pH value of 4 under dark condition.This ERM fungus has adopted well to the relatively low temperature and acidic soil environment of the tundra of Changbai Mountains.Further in-depth investigation and economical utilization of H.hepaticicola and other related ERM fungi will be continued.

关 键 词：欧石南菌根 Hyaloscypha hepaticicola 分子鉴定 生物学特性 

分 类 号：Q949.32[生物学—植物学]