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作 者:赵小红 雷美艳[1] 韩量[1] 蒲潇 韦中强[1] 肖波[1] ZHAO Xiaohong;LEI Meiyan;HAN Liang;PU Xiao;WEI Zhongqiang;XIAO Bo(Chongqing Institute of Medicinal Plant Cultivation,Chongqing 408435,China)
出 处:《菌物学报》2025年第1期61-71,共11页Mycosystema
基 金:重庆市基本科研业务费项目(2022JK006,2023jbky-0013)。
摘 要:杜鹃兰Cremastra appendiculata是珍稀中药材山慈菇基原之一,以假鳞茎入药,其种子细小且不含胚乳,需要合适的共生真菌为其提供营养物质才能完成种子萌发过程。本研究以分离自杜鹃兰根部的共生真菌(DJL1)为研究对象,结合形态特征和内转录间隔区(ITS)序列分析鉴定该真菌为白假小鬼伞Coprinellus disseminatus。从碳源、氮源、pH和温度4个方面对该菌株进行生物学特性研究,并从中选出3个较优水平进行正交试验。结果表明:在试验范围内该菌株生长的最佳碳源为可溶性淀粉,氮源为酵母膏,最适培养温度为25℃,最适pH为7.0。该研究结果为白假小鬼伞真菌资源的保护和开发利用奠定基础。Pseudobulbs of Cremastra appendiculata have been used as precious and rare traditional Chinese medicine.C.appendiculata seed germination depends on mycorrhizal fungi because of lacking endosperm in tiny dust-like seeds under natural conditions.A symbiotic fungus isolate DJL1 obtained from the roots of C.appendiculata was identified as Coprinellus disseminatus on the basis of morphological and molecular biological characteristics.The results of studies of the biological characteristics showed that the optimum carbon source for the mycelial growth of C.disseminatus were soluble starch,the optimum nitrogen source was yeast extract,the optimum pH was 7.0 and the optimum temperature was 25℃.This study was expected to be helpful to the future protection and utilization of C.disseminatus.
分 类 号:S567.239[农业科学—中草药栽培] Q949.32[农业科学—作物学]
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