醛酮还原酶akr基因对糙皮侧耳生长发育的影响  

Effects of aldo-keto reductase akr gene on the growth and development of Pleurotus ostreatus

作　　者：弥春霞[1] 朱晓凯 卢树卓 邬向丽[2] MI Chunxia;ZHU Xiaokai;LU Shuzhuo;WU Xiangli(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang,China;State Key Laboratory of Efficient Utilization of Arid and Semi-arid Arable Land in Northern China,Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011 [2]中国农业科学院农业资源与农业区划研究所,北方干旱半干旱耕地高效利用全国重点实验室,北京100081

出　　处：《菌物学报》2025年第1期83-92,共10页Mycosystema

基　　金：国家自然科学基金(32072643);国家食用菌产业技术体系(CARS20);作物种质资源保护与利用项目。

摘　　要：糙皮侧耳Pleurotus ostreatus子实体发育是一个复杂的生物过程,受体内基因调控及外界各种环境因素的影响。醛酮还原酶(aldo-keto reductase,AKR)在生物生长发育及逆境胁迫响应中起重要作用。本研究对糙皮侧耳一个akr基因在生长发育中的作用进行研究。利用根癌农杆菌介导的转化方法构建糙皮侧耳akr基因的RNA干扰菌株,研究akr基因对糙皮侧耳菌丝生长速率、热胁迫响应及子实体发育的影响。结果显示,与野生型相比,干扰菌株在适温、热激及恢复期间的生长速率均显著降低,原基的形成时间显著延长。研究表明,akr基因促进糙皮侧耳在适温及高温下的菌丝生长、促进原基形成。Fruiting body development of Pleurotus ostreatus is a complex biological process regulated by genes in vivo and influenced by various external environmental factors.Aldo-keto reductases(AKR)play important roles in growth and development and stress responses in organisms.In this study,the role of an akr gene during mycelial growth and fruiting body development in P.ostreatus was revealed.The akr RNA interference strains were constructed by Agrobacterium tumefaciens-mediated transformation method,and the effects of akr gene on the mycelial growth rate,heat stress response,and fruiting body development of P.ostreatus were studied.The results showed that,compared with the wild type,the growth rates of the interference strains were significantly reduced during culturing at optimum temperature,heat shock condition and recovery period.The formation time of the primordia was prolonged considerably.These results revealed that akr gene promoted mycelia growth at normal and high temperature,and primordium formation.

关 键 词：糙皮侧耳 子实体 生长发育 RNA干扰 

分 类 号：S646.14[农业科学—蔬菜学]