祁连山褐色双孢蘑菇的驯化栽培  

Domestication and cultivation of a wild brown strain of Agaricus bisporus collected from Qilian Mountains

作　　者：梁倩倩 丁玲强 田丽雯 王玉臻 牛鑫 单华佳 LIANG Qianqian;DING Lingqiang;TIAN Liwen;WANG Yuzhen;NIU Xin;SHAN Huajia(Gansu Engineering Laboratory of Applied Mycology,Hexi University,Zhangye 734000,Gansu,China;Gansu Key Laboratory of Genetics and Breeding of Edible Mushroom,Hexi University,Zhangye 734000,Gansu,China;College of Life Sciences and Engineering,Hexi University,Zhangye 734000,Gansu,China;Qilian Mountain Fungus Protection and Utilization Collaborative Innovation Base,Hexi University,Zhangye 734000,Gansu,China;College of Agriculture and Ecological Engineering,Hexi University,Zhangye 734000,Gansu,China)

机构地区：[1]河西学院甘肃省应用真菌工程实验室,甘肃张掖734000 [2]河西学院甘肃省食用菌遗传育种重点实验室,甘肃张掖734000 [3]河西学院生命科学与工程学院,甘肃张掖734000 [4]河西学院祁连山菌物保护利用协同创新基地,甘肃张掖734000 [5]河西学院农业与生态工程学院,甘肃张掖734000

出　　处：《菌物学报》2025年第1期116-128,共13页Mycosystema

基　　金：甘肃省科技厅重点研发计划(23YFNG0006);张掖市重大技术攻关揭榜挂帅制项目(ZY2022JBGS07);甘肃省省级重点人才项目(2023RCXM34);甘肃省自然科学基金(23JRRG0031);甘肃省教育厅高校教师创新基金(2024A151)。

摘　　要：本研究对采自祁连山的一株野生菌株进行形态学和分子生物学鉴定,通过单因素试验探讨不同因素对菌丝生长的影响,通过正交试验优化菌丝最优生长配方,进行驯化栽培并对接种量和栽培料进行优化。结果表明:野生菌LQQ2019129鉴定为双孢蘑菇Agaricus bisporus,子实体为褐色;在试验范围内,菌丝生长的最适温度为28℃,最适碳源为蔗糖,最适氮源为酵母粉,最适无机盐为硫酸钙,最适硫酸钙浓度为1.6 g/L;菌丝生长的最优培养基配方为马铃薯200 g/L,蔗糖15 g/L,酵母粉4 g/L,硫酸钙1.6 g/L,水1000 mL,pH自然;子实体在麦草和玉米秸秆上均可出菇,最适接菌量为麦草5%,玉米秸秆10%,麦草较玉米秸秆更适于该菌株的栽培。Based on morphological and molecular identification,a wild fungal strain LQQ2019129 with brown fruiting bodies collected from the Qilian Mountains was identified as Agaricus bisporus.The effects of different factors on mycelial growth were explored through single factor experiments,and the optimal culture medium formula for domestication and cultivation was optimized through orthogonal experiments.The results showed that,the optimal temperature for mycelial growth is 28℃,the optimal carbon source is sucrose,the optimal nitrogen source is yeast powder,the optimal inorganic salt is calcium sulfate,and the optimal calcium sulfate concentration is 1.6 g/L;the optimal medium formula is potato 200 g/L,sucrose 15 g/L,yeast powder 4 g/L,calcium sulfate 1.6 g/L,water 1000 mL.Under natural pH,the mushroom can produce fruiting body on both wheat straw and corn straw as cultivation substrate,with optimal inoculation amount of 5%for wheat straw and 10%for corn straw.Wheat straw is more suitable than corn straw.

关 键 词：形态学鉴定 ITS鉴定 生物学特性 驯化栽培 

分 类 号：S646.11[农业科学—蔬菜学]