小檗碱与氟康唑合用抗氟康唑耐受白念珠菌的研究  

Study on the effect of berberine combined with fluconazole on fluconazole-tolerant Candida albcians strains

作　　者：宋泽成 马闪闪 胡巧灵 仲华 王彦 SONG Zecheng;MA Shanshan;HU Qiaoling;ZHONG Hua;WANG Yan(School of Pharmacy,Naval Medical University,Shanghai 200433,China;School of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;The Center for Fungal Infectious Diseases Basic Research and Innovation of Medicine and Pharmacy,Ministry of Education,Shanghai 200433,China)

机构地区：[1]海军军医大学药学系,上海200433 [2]福建中医药大学药学院,福建福州350122 [3]真菌感染性疾病教育部医药基础研究创新中心,上海200433

出　　处：《药学实践与服务》2025年第2期87-91,共5页Journal of Pharmaceutical Practice and Service

基　　金：生物安全项目(145AHQ082021000X);国家自然科学基金(82204470)。

摘　　要：目的研究小檗碱(BBR)与氟康唑(FLC)联合用药的体外抗FLC耐受白念珠菌作用。方法利用微量液基稀释法测定FLC单用对8株白念珠菌的最低抑菌浓度(MIC)以确定其对FLC的敏感性;通过琼脂平皿纸片扩散实验从FLC敏感菌株中筛选出FLC耐受菌株;利用琼脂平皿纸片扩散实验考察BBR与FLC联合用药对FLC耐受白念珠菌的作用。结果选取的8株白念珠菌皆为FLC敏感菌株,其MIC50值均<0.5μg/ml;菌株Y0109、9821、7879、7654、9296在恒温培养48 h后抑菌圈内出现菌落生长,表现出对FLC的耐受现象;菌株Y0109与9821在BBR与FLC联用时,恒温培养48 h后抑菌圈内的菌落数随BBR浓度的升高而逐渐减少,抑菌圈随BBR浓度的升高而逐渐清晰,随FLC载药量的增大而增大,显示出剂量依赖关系。结论BBR与FLC联合用药具有良好的抗FLC耐受白念珠菌效果。Objective To investigate the combined effect of berberine(BBR)and fluconazole(FLC)on FLC-tolerant Candida albicans in vitro.Methods The sensitivity of 8 strains of Candida albicans to FLC was assessed by determining their minimal inhibitory concentration(MIC)using broth microdilution method.FLC-tolerant strains were screened from FLC-sensitive strains by disk diffusion assay.The effect of BBR combined with FLC on FLC-tolerant Candida albicans was investigated by disk diffusion assay.Results All eight strains of Candida albicans exhibited sensitivity to FLC,with minimal inhibitory concentration(MIC50)values below 0.5μg/ml.Strains Y0109,9821,7879,7654,and 9296 displayed colony growth in the inhibition zone after 48 h of constant temperature incubation,indicating FLC tolerance.When strains Y0109 and 9821 were subjected to a combination of BBR and FLC,the number of colonies within the inhibition zone decreased progressively with the increase of BBR concentration following a 48 h constant temperature culture.The inhibition zone became clear with the increasing of BBR concentration and increased with the increase of FLC loading,which showed a dose-dependent relationship.Conclusion The BBR combined with FLC demonstrated efficacy against FLC-tolerant strains.

关 键 词：白念珠菌 小檗碱 氟康唑 联合用药 药物耐受 

分 类 号：R44[医药卫生—诊断学]