基于网络药理学探索苗药大血藤-飞龙掌血治疗糖尿病认知障碍的分子作用机制  

Investigating the molecular mechanism of Daxueteng-Feilongzhangxue Herbal Pair in treating diabetic cognitive impairment based on network pharmacology

作　　者：娄方丽 田辉 罗洁 吴远华 LOU Fangli;TIAN Hui;LUO Jie;WU Yuanhua(Nursing College,Guizhou University of Traditional Chinese Medicine,Guizhou 550025 China)

机构地区：[1]贵州中医药大学护理学院,550025 [2]贵州中医药大学人文与管理学院,550025 [3]贵州中医药大学体育健康学院,550025 [4]贵州中医药大学第一附属医院神内科,550001

出　　处：《全科护理》2025年第4期664-674,共11页Chinese General Practice Nursing

基　　金：贵州省中医药管理局中医药、民族医药科学技术研究专项项目,编号:QZYY-2021-068。

摘　　要：目的:基于网络药理学研究苗药大血藤-飞龙掌血药对治疗糖尿病认知障碍(DCI)的分子作用机制。方法:基于TCMSP、HERB、PubChem等数据库筛选靶点基因,利用Venny 2.1.0软件取其交集,利用Cytoscape v3.9.1软件创建其药物-活性成分-基因靶点网络,使用STRING数据库建立蛋白相互作用(PPI)网络图,使用DAVID数据库和微生信进行GO功能富集分析及KEGG通路富集分析,使用Cytoscape v3.9.1软件建立靶点-通路网络图。结果:大血藤和飞龙掌血的有效成分分别为33个、21个,共同活性成分2个。药物-疾病交集靶点基因275个。核心基因包括ALB、AKT1、SRC、TNF、PPARG、PRKACA、EGFR、ESR1、APP、BCL2、MAPK3、PTGS2、STAT3、CASP3、GRIN2B、GSK3β、HSP90AA1、DRD2、HIF1A、NFKB1等。GO功能富集分析932个,包括磷酸化、对外源性刺激的反应、信号传导、凋亡过程的负调节、对缺氧/脂多糖的反应等。KEGG通路190个,集中在人类疾病、生物学及环境信息过程,包括糖尿病并发症、癌症、病毒感染、动脉粥样硬化、内分泌抵抗及AGE-RAGE、HIF-1、PI3K/Akt、催乳素、Ras、Relaxin信号通路等。结论:大血藤和飞龙掌血治疗DCI的共同活性成分为β-谷甾醇、绿原酸。药对通过调节ALB、AKT1、SRC、TNF、PPARG、PRKACA等靶点基因,调控糖尿病并发症、癌症、病毒感染、动脉粥样硬化、内分泌抵抗及AGE-RAGE、HIF-1、PI3K/Akt信号通路等实现抗癌、治疗糖尿病并发症、抗病毒感染、改善血液流变学、改善内分泌和代谢等,发挥治疗DCI作用。Objective:To investigate the molecular mechanism of action of the traditional Chinese medicine(TCM) pair of DaxuetengFeilongzhangxue in the treatment of diabetic cognitive impairment(DCI) based on network pharmacology.Methods:The target genes were screened based on TCMSP,HERB,and PubChem databases,and the intersection of the target genes was obtained using Venny 2.1.0.Cytoscape v3.9.1 was used to create a drug-active component-gene target network,and the PPI network diagram was established using the STRING database.DAVID database and MicroHIS were used for GO function enrichment analysis and KEGG pathway enrichment analysis,and Cytoscape v3.9.1 was used to establish a target-pathway network diagram.Results:The effective components of Daxueteng and Feilongzhangxue were 33 and 21,respectively,with a common active component of 2.The drug-disease intersection target genes were 275.The core genes included ALB,AKT1,SRC,TNF,PPARG,PRKACA,EGFR,ESR1,APP,BCL2,MAPK3,PTGS2,STAT3,CASP3,GRIN2B,GSK3β,HSP90AA1,DRD2,HIF1A,and NFKB1.The GO function enrichment analysis identified 932terms,including phosphorylation,response to exogenous stimuli,signal transduction,negative regulation of apoptotic process,and response to hypoxia/lipopolysaccharide.The KEGG pathways were 190,concentrated in human diseases,biological and environmental information processes.They included diabetes complications,cancer,viral infections,atherosclerosis,endocrine resistance,and AGE-RAGE,HIF-1,PI3K/Akt,prolactin,Ras,Relaxin signaling pathways,etc.Conclusion:The common active constituents of Daxueteng and Feilongzhangxue in treating DCI are β-sitosterol and chlorogenic acid.The drug pair regulates target genes such as ALB,AKT1,SRC,TNF,PPARG,PRKACA,etc.by adjusting the AGE-RAGE,HIF-1,PI3K/Akt signaling pathways,cancer,diabetes complications,viral infections,atherosclerosis,endocrine resistance,and other pathways to achieve anti-cancer,treatment of diabetes complications,anti-viral infections,improvement of hemorheology,improvement of endocrine and metabolism,

关 键 词：大血藤 飞龙掌血 网络药理学 糖尿病认知障碍 分子作用机制 

分 类 号：R29[医药卫生—民族医学]