基于光合和叶绿素荧光特性的九牛草种质资源评价  

Germplasm resource evaluation of Artemisia stolonifera based on photosynthetic and chlorophyll fluorescence characteristics

作　　者：王业 王楚 常娜娜 徐扬 黄璐琦[3] 李慧[1,2,4] WANG Ye;WANG Chu;CHANG Na-na;XU Yang;HUANG Lu-qi;LI Hui(Institute of Traditional Chinese Medicine Health Industry,China Academy of Chinese Medical Sciences/Jiangxi Key Laboratory for Sustainable Utilization of Chinese Materia Medica Resources,Nanchang 330115,China;Jiangxi Institute of Traditional Chinese Medicine Health Industry,Nanchang 330115,China;China Academy of Chinese Medical Sciences,Beijing 100700,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中医药健康产业研究所/中药资源可持续利用江西省重点实验室,南昌330115 [2]江西中医药健康产业研究院,南昌330115 [3]中国中医科学院,北京100700 [4]中国中医科学院中药研究所,北京100700

出　　处：《湖北农业科学》2025年第1期81-87,共7页Hubei Agricultural Sciences

基　　金：中国中医科学院科技创新工程项目(CI2021A05204);江西省重大科技研发专项“揭榜挂帅”关键技术类项目(20223AAF01007);赣江新区科技计划项目(2021010)。

摘　　要：为筛选高产稳产的九牛草(Artemisia stolonifera)优良种质资源,对不同表型九牛草叶片光合荧光参数进行评价。以江西省樟树市孙家村种植的九牛草为试验材料,以6种表型九牛草作为测试对象,采用便携式光合仪测定其叶绿素光合荧光参数,并结合方差分析、主成分分析和隶属函数分析方法,综合评价不同表型九牛草光合能力和潜在的适应性。结果表明,6种九牛草表型叶片形态差异明显,不同表型叶片光合参数和叶绿素荧光参数均存在显著差异,深绿大叶型[(16.260±3.585)μmol/(m^(2)·s)]和浅绿狭叶型[(17.366±3.982)μmol/(m^(2)·s)]净光合速率显著高于其他4种表型。主成分分析表明,净光合速率、胞间二氧化碳浓度、水汽压亏缺值、初始荧光值和暗适应下最大荧光值均为重要贡献变量。隶属函数值计算结果表明,浅绿狭叶型(0.58)、深绿大叶型(0.53)、深绿狭叶型(0.50)3种表型综合排序得分较高,适合作为候选的优良种质。In order to select high and stable germplasm resources of Artemisia stolonifera,photosynthetic fluorescence parameters of leaves of different phenotypes of Artemisia stolonifera were evaluated.Artemisia stolonifera planted in Sunjia Village,Zhangshu City,Jiangxi Province was used as experimental material,and six phenotypes of Artemisia stolonifera were used as test objects.The chloro-phyll photosynthetic fluorescence parameters were determined by portable photosynthesometer,and the photosynthetic capacity and potential adaptability of different phenotypes were evaluated by variance analysis,principal component analysis and membership func-tion analysis.The results showed that the phenotypic leaf morphology of the 6 species was significantly different,and the photosynthet-ic parameters and chlorophyll fluorescence parameters of the leaves of different phenotypes were significantly different.The net photo-synthetic rates of dark green large leaf type[(16.260±3.585)μmol/(m^(2)s)]and light green narrow leaf type[(17.366±3.982)μmol/(m^(2)s)]were significantly higher than those of the other 4 phenotypes.Principal component analysis showed that net photosynthetic rate,intercellular carbon dioxide concentration,water vapor pressure deficit value,initial fluorescence value and maximum fluores-cence value under dark adaptation were important contributing variables.The results of membership function value calculation showed that light green narrow leaf type(0.58),dark green large leaf type(0.53)and dark green narrow leaf type(0.50)had higher compre-hensive ranking scores and were suitable as candidate excellent germplasm.

关 键 词：九牛草(Artemisia stolonifera) 光合参数 叶绿素荧光 种质资源 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]