玉米磷脂酶A基因家族鉴定及其在盐胁迫条件下的差异表达分析  

Identification of Maize Phospholipase A Gene Family and Analysis of Its Differential Expression under Salt Stress

作　　者：李慧欣 车致远 王旭 解艳萍 王璐 谷心如 车欣洋 张海洋 邓杰 魏金鹏 贺琳 魏玉磊 徐晶宇 LI Hui-xin;CHE Zhi-yuan;WANG Xu;XIE Yan-ping;WANG Lu;GU Xin-ru;CHE Xin-yang;ZHANG Hai-yang;DENG Jie;WEI Jin-peng;HE Lin;WEI Yu-lei;XU Jing-yu(College of Agriculture,Heilongjiang Bayi Land Reclamation University,Heilongjiang Province Key Laboratory of Modern Agricultural Cultivation Technology and Crop Germplasm Improvement,Daqing 163319,China)

机构地区：[1]黑龙江八一农垦大学农学院/黑龙江省现代农业栽培技术与作物种质改良重点实验室,黑龙江大庆163319

出　　处：《玉米科学》2025年第1期14-21,共8页Journal of Maize Sciences

基　　金：黑龙江省自然基金重点项目(ZD2020C007);黑龙江八一农垦大学三横三纵重点专项(ZDZX202101);黑龙江八一农垦大学研究生创新项目(YJSCX2022-Y08)。

摘　　要：磷脂酶A(phospholipase A,PLA)在植物生长发育、脂信号转导以及胁迫响应方面发挥着重要作用。在玉米全基因组中共鉴定出38个编码PLA蛋白基因,并对其进化关系、基因结构、不同组织及盐胁迫下的表达模式等进行综合分析。根据玉米PLA家族成员的序列同源性和进化关系可分为PLA1(phosphipase A1)、pPLA(patatin like phosphipase A)和sPLA2(secretory Phospholipases A2)3个亚家族。基因表达分析发现,不同成员在不同组织部位的表达模式各不相同,ZmPA-PLA1α、ZmPA-PLA1β与ZmpPLA-Iα、ZmpPLA-Iβ在整个生长发育阶段的相对表达量都是较高的。对盐胁迫(150 mmol/L NaCl)处理下玉米幼苗转录组数据进行分析,发现ZmPLA1-Iγ2在根和叶片中均上调表达,ZmPLA1-Iα1、ZmPLA1-Iα2、ZmPLA1-Iα3、ZmPLA1-Iβ1在根中上调表达,推测PLA基因在盐胁迫响应中具有重要作用。Phospholipase A(PLA)plays an important role in plant growth and development,lipid signal transduction,and stress responses.In this study,a total of 38 genes encoding PLA proteins were identified in the maize genome,and their evolutionary relationships,gene structures,expression patterns in different tissues and under salt stress were comprehensively analyzed.Based on the sequence homology and evolutionary relationships,the maize PLA family members were categorized into three subfamilies:PLA1(photosphorase A1),pPLA(patatin like photosphorase A),and sPLA2(secret photosphoripases A2).Gene expression analysis revealed that the expression of different PLA members vary in different tissue.The relative expression level of ZmPA-PLA1α,ZmPA-PLA1βAnd ZmpPLAIα,ZmpPLA-Iβis relatively high throughout the entire growth and development stage.The transcriptome data of maize seedlings under salt stress(150mmol/L NaCl)treatment was analyzed,it was found that ZmPLA1-Iγ2 was upregulated in both roots and leaves,while ZmPLA1-Iα1,ZmPLA1-Iα2,ZmPLA1-Iα3,and ZmPLA1-Iβ1 were up-regulated in roots,which suggesting that PLA gene plays an important role in salt stress responses.

关 键 词：玉米 磷脂酶A 生物信息学 基因表达 盐胁迫 

分 类 号：S513.035.3[农业科学—作物学]