靶向微泡破坏技术可逆性破坏血睾屏障  

Targeted microbubble disruption technology can reversibly destroy the blood-testicular barrier

作　　者：何养妙 朱慧敏[1] 冯健一 国晓雯 王璐瑶[1] 顾朝辉[1] 陈争光[1] He Yangmiao;Zhu Huimin;Feng Jianyi;Guo Xiaowen;Wang Luyao;Gu Chaohui;Chen Zhengguang(Department of Urology,the first Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院泌尿外科,郑州450052

出　　处：《中华实验外科杂志》2024年第12期2713-2716,共4页Chinese Journal of Experimental Surgery

基　　金：河南省科技攻关计划(212102310139);河南省高等学校重点科研项目计划(25A320059)。

摘　　要：目的探讨超声靶向微泡破坏技术(UTMD)对大鼠睾丸血睾屏障的影响。方法以48只成年雄性SD大鼠为实验对象,在不同的实验条件下随机(随机化生成器法)将大鼠分为对照组,超声(US)组,超声+低浓度微泡(US+LM)组,超声+高浓度微泡(US+HM)组。对紧密连接蛋白(Occuldin、ZO1)、肌动蛋白调节蛋白(Formin1、Arp3)微管蛋白调节蛋白(EB1)进行免疫蛋白印迹检测,用免疫荧光技术检测细胞骨架蛋白(F-actin)在生精小管中的分布,观察6 h后血睾屏障组分蛋白质数量和分布的变化。通过生物素扩散距离(D)与生精小管半径(R)的比值×100%,半定量血睾屏障损伤程度。两组间比较采用t检验,多组间比较采用单因素方差分析。结果D/R比值,对照组为0%;US组为(37.97±0.32)%;US+LM组为(80.70±1.70)%;US+HM组为(98.33±1.44)%(US组比US+LM组,t=-42.639,P<0.01;US组比US+HM组,t=-71.033,P<0.01;US+LM组比US+HM组,t=-13.695,P<0.01);F-actin,对照组:呈长直条状,方向从基膜指向生精小管中心;US组:呈轻度弯曲条状,方向稍偏离基膜到生精小管中心;US+LM组:呈中度弯曲条状,方向明显偏离基膜到生精小管中心;US+HM组:断裂短片状,不具有方向性;EB1/β-肌动蛋白(β-actin),对照组:(0.95±0.01);US组:(0.72±0.02);US+LM组:(0.54±0.09);US+HM组:(0.44±0.01)(对照组比US组,t=-20.602,P<0.01;对照组比US+LM组,t=-7.719,P<0.01;对照组比US+HM组,t=-57.386,P<0.01)。结论UTMD可能通过EB1、F-actin介导使睾丸屏障可逆性破坏。ObjectiveTo explore the effect of ultrasound-targeted microbubble disruption technology(UTMD)on the blood-testis barrier of rat testis.MethodsForty-eigg adult male SD rats were randomly(randomization generator method)divided into control,ultrasound(US),ultrasound+low concentration of microbubbles(US+LM),and ultrasound+high concentration of microbubbles(US+HM)groups under different experimental conditions.Immunoprotein blotting for tight junction protein(Occuldin,ZO1),actin regulatory protein(Formin1,Arp3)microtubule protein regulatory protein(EB1),and cytoskeletal protein(F-actin)expression in spermatocytes were detected by immunocytofluorescence,and the changes in the number and distribution of blood-testis barrier component proteins were observed after 6 h.The degree of BTB damage was semi-quantified by the ratio of biotin diffusion distance(D)to the radius of the seminiferous tubules(R)×100%.Comparisons between two groups were performed by t-test,and comparisons between multiple groups were performed by one-way ANOVA.ResultsD/R ratio,control group:0%;US group:(37.97±0.32)%;US+LM group:(80.70±1.70)%;US+HM group:(98.33±1.44)%(US group vs.US+LM group,t=-42.639,P<0.01;US group vs.US+HM group,t=-71.033,P<0.01;US+LM group vs.US+HM group,t=-13.695,P<0.01).F-actin:control group:long straight strip,oriented from basement membrane to the center of seminiferous tubules;US group:mildly curved strip,oriented slightly away from basement membrane to the center of seminiferous tubules;US+LM group:moderately curved strip,oriented significantly away from basement membrane to the center of seminiferous tubules;US+HM group:broken short sheet,not oriented.EB1/β-actin:control group:(0.95±0.01);US group:(0.72±0.02);US+LM group:(0.54±0.09);US+HM group:(0.44±0.01)(control group vs.US group,t=-20.602,P<0.01;control group vs.US+LM group,t=-7.719,P<0.01;control group vs US+HM group,t=-57.386,P<0.01).ConclusionUTMD may mediate the reversible disruption of testicular barrier through EB1,F-actin.

关 键 词：血睾屏障 超声靶向微泡破坏技术 睾丸 

分 类 号：R697.22[医药卫生—泌尿科学]