A biomimetic magnetically responsive scaffold with tunable and stable compression for dynamic 3D cell culture  

一种用于动态三维细胞培养的具有可调控稳定压缩的仿生磁响应支架

作　　者：Xiao Sun Xiaohong Wang Bingjie Wu Qianhong Yang Congxiao Zhu Huimin Zhang Qian Li Hongru Zhou Minghui Guo Lin Gui Lei Li 孙晓;汪晓红;武冰洁;杨乾泓;朱丛笑;张慧敏;李倩;周宏儒;郭明晖;桂林;李雷(Key Laboratory of Cryogenic Science and Technology,Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,China;School of Biomedical Engineering,Tsinghua University,Beijing 100084,China;Plastic Surgery Hospital,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100144,China;School of Future Technology,University of Chinese Academy of Sciences,Beijing 100039,China)

机构地区：[1]Key Laboratory of Cryogenic Science and Technology,Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,China [2]School of Biomedical Engineering,Tsinghua University,Beijing 100084,China [3]Plastic Surgery Hospital,Chinese Academy of Medical Sciences,Peking Union Medical College,Beijing 100144,China [4]School of Future Technology,University of Chinese Academy of Sciences,Beijing 100039,China

出　　处：《Science China Materials》2025年第2期652-665,共14页中国科学(材料科学)(英文版)

基　　金：supported by the CAMS Innovation Fund for Medical Sciences (2021-I2M-1-052)。

摘　　要：Magnetically responsive scaffolds are extensively utilized in tissue engineering for their ability to simulate dynamic three-dimensional(3D)cell microenvironment in a rapid,reversible,and contactless manner.However,existing magnetic scaffolds struggle to provide tunable dynamic compression comparable to natural tissues due to the weak magnetism of magnetic nanoparticles and the mechanical brittleness of hydrogels.Here,we propose a biomimetic 3D magnetic scaffold offering tunable and stable magnetically induced compression for dynamic 3D cell culture.By employing hard magnetic particles NdFeB@SiO_(2) and a mechanically stable elastomer,Ecoflex,the scaffold achieves 15%compression in the magnetic field(240 mT).Moreover,this magnetic scaffold demonstrates remarkable deformation and mechanical stability during 4000 compression cycles.The magnetic scaffold exhibits stiffness(0.78 kPa)and viscoelasticity(relaxation time of 17 s)similar to adipose tissue.Notably,it is verified that human adipose-derived stem cells(hADSCs)are successfully cultured in this magnetic scaffold and the proliferation of hADSCs can be modulated by magnetically induced dynamic compression.This magnetic scaffold for dynamic 3D cell culture can be potentially utilized in cell biology and tissue engineering.磁响应支架能以快速、可逆和非接触的方式模拟体内细胞生长的动态三维微环境,因此被广泛用于组织工程领域.然而,受限于磁性纳米颗粒的弱磁性和水凝胶的机械脆性,现有的磁性支架很难为细胞提供与天然组织相似的动态压缩环境.为解决这一问题,我们提出了一种用于动态三维细胞培养的仿生磁性支架,为细胞提供可调且稳定的磁致压缩刺激.由硬磁颗粒NdFeB@SiO_(2)和机械稳定的弹性体Ecoflex组成的磁性支架在磁场中(240 mT)可以产生高达15%的压缩应变.而且,磁性支架在4000次压缩循环中表现出优异的变形和机械稳定性.磁性支架还具有与脂肪组织相似的刚度(0.78 kPa)和粘弹性(弛豫时间为17 s).实验表明,这种磁性支架不仅成功实现了人脂肪干细胞(hADSCs)的体外三维培养,还可以通过磁致压缩调节hADSCs的增殖.这种实现了动态三维细胞培养的磁性支架未来可能被应用于细胞生物学和组织工程研究.

关 键 词：magnetically responsive scaffolds dynamic 3D cell culture adipose-derived stem cells cell proliferation cyclic compression 

分 类 号：TG1[金属学及工艺—金属学]