新冠病毒变异株基于核酸扩增检测方法研究进展  

Nucleic acid amplification-based detection methods for SARS-CoV-2 variant detection:research progress review

作　　者：伍霞 张晓[1] 韩嘉艺[1] 邢方潇[1] 叶必雄[1] WU Xia;ZHANG Xiao;HAN Jiayi;XING Fangxiao;YE Bixiong(Water Quality and Health Monitoring Laboratory,National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases,Key Laboratory of Environment and Population Health,Chinese Center for Disease Control and Prevention,Beijing 10050,China)

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所、水质量与健康监测室传染病溯源预警与智能决策全国重点实验室、中国疾病预防控制中心环境与人群健康重点实验室,北京100050

出　　处：《中国公共卫生》2024年第11期1396-1400,共5页Chinese Journal of Public Health

基　　金：国家重点研发计划(2023YFC3041300–02)。

摘　　要：新型冠状病毒(SARS-CoV-2)引起的新冠病毒感染疫情仍在全球范围蔓延,SARS-CoV-2易发生变异,导致其变异株传播性、致病性和免疫逃逸能力增强,对全球造成不可忽视的影响。为有效监测新型冠状病毒感染,需研发针对变异株准确、灵敏或现场适用的多样化检测技术。本文阐述了SARS-CoV-2变异株的基于核酸扩增的检测技术原理及在识别和区分变异株方面的应用,探讨了方法的优缺点,对于准确发现并高效检测SARS-CoV-2变异株提供参考。The coronavirus disease 2019(COVID-19)pandemic,caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),continues to spread worldwide.SARS-CoV-2 is prone to mutation,resulting in variants with increased transmissibility,pathogenicity,and immune escape capabilities,posing a significant global threat.To effectively monitor new coronavirus infections,it is necessary to develop diverse detection technologies that are accurate,sensitive,or field-usable for variant identification.This article describes the principles of nucleic acid amplification-based detection techniques for SARS-CoV-2 variants and their applications variant identification and differentiation.It also discusses the advantages and disadvantages of these methods and provides a reference for the accurate identification and efficient detection of SARS-CoV-2 variants.

关 键 词：新型冠状病毒 变异株 核酸扩增技术 

分 类 号：R511[医药卫生—内科学] R440[医药卫生—临床医学]