Fluorescence sensor based on IFE between AEP@UCNPs and oxTMB for the determination of Cd^(2+)in paddy rice combined with HRP enzyme inhibition mechanism  

作　　者：Haizhen Ding Shuai Hou Siqi Zhao Liping Guo Ao Sun Qiuhui Hu Leiqing Pan Qiang Liu Chao Ding 

机构地区：[1]Collaborative Innovation Center for Modern Grain Circulation and Safety,Key Laboratory of Grains and Oils Quality Control and Processing,College of Food Science and Engineering,Nanjing University of Finance and Economics,Nanjing 210023,China [2]College of Food Science and Technology,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Food Science and Human Wellness》2025年第1期154-160,共7页食品科学与人类健康(英文)

基　　金：financially supported by the National Natural Science Foundation of China(32202132,32172229);Young Elite Scientists Sponsorship Program by CAST(2022QNRC001);the Priority Academic Program Development of Jiangsu Higher Educations(PAPD)。

摘　　要：Cadmium ion(Cd^(2+))detection technology plays a prominent role in food safety and human health.Herein,we designed and constructed an 2-aminoethyl dihydrogen phosphate(AEP)@upconversion nanoparticles(UCNPs)fluorescence sensor for quantitative detection of Cd^(2+)in paddy rice based on inner filter effect(IFE)combined with enzyme inhibition mechanism.The AEP modification UCNPs can offer a stable fluorescence donor at 658 nm and be quenched by the oxidized tetramethylbenzidine(oxTMB)catalyzed by horseradish peroxidase(HRP)enzymes.Without addition of Cd^(2+),the fluorescence of AEP@UCNPs fluorescence sensor was weaken due to the IFE between AEP@UCNPs and oxTMB.With addition of Cd^(2+),HRP enzyme activity was inhibited by Cd^(2+),leading to the decreased oxTMB,resulting in the enhance upconversion fluorescence intensity.As a result,the fluorescence intensity signal at 658 nm of the IFE-based AEP@UCNPs fluorescence sensor increased linearly with the increase in Cd^(2+)in a wide range from 0.5μmol/L to 6μmol/L and the limit of detection(LOD)was 24.6 n mol/L.In addition,our proposed IFE-based AEP@UCNPs fluorescence sensor can achieve Cd^(2+)detection in paddy rice in 30 min.

关 键 词：Paddy rice Cd^(2+) Enzyme inhibition Upconversion nanoparticles(UCNPs) Inner filter effect(IFE) 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置] R155.5[自动化与计算机技术—控制科学与工程]