Anthocyanin attenuates disturbance of intestinal barrier in high fat-high cholesterol diet-challenged mice through regulating the response of T helper 17 cells  

作　　者：Qiannan Liu Juan Pang Yi Tang Yiran You Jiaxin Mi Jinghe Xiao Yu Chen Wenhua Ling 

机构地区：[1]Department of Nutrition,School of Public Health,Sun Yat-sen University,Guangzhou 510000,China [2]Laboratory of Clinical Pharmacy and Adverse Drug Reaction,West China Hospital,Sichuan University,Chengdu 610000,China [3]Department of Nutrition,The First People's Hospital of Zhaoqing,Zhaoqing 526000,China [4]Guangdong Provincial Key Laboratory of Food,Nutrition and Health,Guangzhou 510000,China

出　　处：《Food Science and Human Wellness》2025年第1期332-344,共13页食品科学与人类健康(英文)

基　　金：supported by the National Natural Science Foundation of China(81973022 and 81730090)。

摘　　要：Anthocyanin,as a typical food bioactive molecule,is capable of reversing inflammatory,oxidative and allergic condition thus contributes to intestinal health.We were wondering whether anthocyanin has influence on the infiltration of inflammatory cells into the intestinal mucosa and thus help enhancing intestinal barrier which could be damaged in some metabolic diseases.In this study,the influence of anthocyanin(administered orally)on the alterations(including structure and permeability)of the intestinal mucosa in mice in response to a high fat-high cholesterol(HFHC)diet was investigated.Primary T helper 17(Th17)cells were isolated from mouse intestine tissues to observe the modulatory role of anthocyanin through the transcription phosphorylated STAT 3(p-STAT3).The results indicated that anthocyanin significantly alleviated HFHC-induced impairment in the intestinal structures and permeability in a dose-dependent manner;moreover,anthocyanin appeared to inhibit HFHC induced the expression of p-STAT3,thereby disturbing Th17 cell differentiation.In high-fat diet(HFD,cholesterol level non-modified)-challenged mice selective p-STAT3 inhibitor significantly reversed the effects of anthocyanin,which were decreased amount of interleukin(IL)-17A(produced and released from Th17 cells)and the protected intestinal structure/function.In summary,the results of this study suggest that anthocyanin may attenuate the damage of intestinal barrier in HFHC mice through regulating intestinal STAT3-Th17-IL-17A signal transduction pathway.

关 键 词：ANTHOCYANIN Intestinal barrier dysfunction STAT3 T helper 17(Th17)cell interleukin(IL)-17A 

分 类 号：TS201.4[轻工技术与工程—食品科学] R574[轻工技术与工程—食品科学与工程]