淀粉基凝胶包封药物复合物制备及清除牙菌斑性能  

Preparation of starch-based drug-loaded nanogel and its effect on dental plaque biofilm removal

作　　者：张玉媛 洪流 杨成 ZHANG Yuyuan;HONG Liu;YANG Cheng(School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,Jiangsu,China;Key Laboratory of Synthetic and Biological Colloids,Ministry of Education,Wuxi 214122,Jiangsu,China)

机构地区：[1]江南大学化学与材料工程学院,江苏无锡214122 [2]合成与生物胶体教育部重点实验室,江苏无锡214122

出　　处：《精细化工》2025年第2期402-410,共9页Fine Chemicals

基　　金：江苏省自然科学基金项目(BK2017175)。

摘　　要：为提升盐酸米诺环素(MH)对牙菌斑的清除效果,利用羧甲基淀粉(CMS)与Sn^(2+)物理交联制备纳米凝胶(CSN),并通过复配壳聚糖(CS)增强靶向黏附性,最后包封MH制备了凝胶包封药物复合物(CS/CSN@MH)。通过1HNMR、FTIR、粒径和Zeta电位、TEM表征了CS/CSN@MH结构组成及微观形貌。测试了CS/CSN@MH的药物包封率、载药率,并考察了其抗菌活性、生物相容性。结果表明,CS/CSN@MH的MH包封率和载药率分别可达76.48%和10.64%;CS/CSN@MH在48 h内的MH累积释放率为47.46%。经CS/CSN@MH处理过后的变形链球菌生物膜残留量仅为13.76%,抗菌性优于游离MH处理的(69.87%),并通过活/死菌染色及SEM技术进一步验证了材料的抗菌性能。细胞毒性实验结果表明,CS/CSN@MH处理过的人牙龈成纤维细胞的存活率>90%,说明材料具备较好的生物相容性。To improve the dental plaque removal efficacy of minocycline hydrochloride(MH),the gel-encapsulated drug complex(CS/CSN@MH)was prepared by encapsulation of MH with nanogel(CSN)synthesized from physical cross-linking of carboxymethyl starch(CMS)and Sn^(2+),which was further compounded with chitosan(CS)for enhancement in targeted adhesion,and characterized by 1HNMR,FTIR,particle size and Zeta potential as well as TEM.The encapsulation rate and drug loading rate of CS/CSN@MH were measured,while its antibacterial activity and biocompatibility were further analyzed.The results showed that encapsulation rate and drug loading rate were 76.48%and 10.64%,respectively.The cumulative MH release rate from CS/CSN@MH within 48 h was 47.46%.After CS/CSN@MH treatment,the residual amount of Streptococcus mutans biofilm was only 13.76%,higher than that by free MH treatment(69.8%).The antibacterial performance of the material were further verified by live/dead bacteria staining and SEM technology.The results from cytotoxicity experiment displayed that the survival rate of human gingival fibroblasts treated with CS/CSN@MH was>90%,indicating that good cytocompatibility of the material.

关 键 词：牙菌斑生物膜 缓释 羧甲基淀粉 纳米凝胶 氟化亚锡 壳聚糖 淀粉化学品 

分 类 号：TQ460.4[化学工程—制药化工]