Efficient gene disruption in polyploid genome by Cas9–Trex2 fusion protein  

作　　者：Wenbo Pan Chunlei Gao De Niu Jinghua Cheng Jiao Zhang Xiying Yan Qiang Long Yao Yao Zhu Wenjing Sun Qi Xie Yuehui He Xing Wang Deng Huawei Zhang Jian Li 

机构地区：[1]State Key Laboratory of Wheat Improvement,Shandong Laboratory of Advanced Agricultural Sciences in Weifang,Peking University Institute of Advanced Agricultural Sciences,Weifang 261325,China [2]Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,Ministry of Education&Guangdong Provincial Key Laboratory of Laser Life Science,School of Life Sciences,South China Normal University,Guangzhou 510631,China [3]Shenzhen Tidy field System Biotechnology Co.,Ltd,Shenzhen 518107,China [4]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,The Innovative Academy of Seed Design,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Journal of Integrative Plant Biology》2025年第1期7-10,共4页植物学报(英文版)

基　　金：supported by the Natural Science Foundation of Shandong Province(ZR2021ZD30,ZR2022ZD22);the Key R&D Program of Shandong Province(ZR202211070163);Taishan Scholar Foundation of Shandong Province(TSQN202103160);the Excellent Youth Foundation of Shandong Scientific Committee(ZR202103010168)。

摘　　要：Despite significant advancements in the efficiency of the CRISPR/Cas9 genome editing system over the past decade,performing gene disruption in polyploid crops such as common wheat(Triticum aestivum L.)remains challenging,especially when trait stacking requires multiplex gene disruption.

关 键 词：CRISPR/Cas9 CROPS TRAIT 

分 类 号：S51[农业科学—作物学]