The PtobZIP55–PtoMYB170 module regulates the wood anatomical and chemical properties of Populus tomentosa in acclimation to low nitrogen availability  

作　　者：Jiangting Wu Shurong Deng Yang Wang Chenlin Jia Jia Wei Mengyan Zhou Dongyue Zhu Zhuorong Li Payam Fayyaz Zhi-Bin Luo Jing Zhou Wenguang Shi 

机构地区：[1]State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China [2]Forest,Range and Watershed Management Department,Agriculture and Natural Resources Faculty,Yasouj University,Yasuj 7591963179,Iran [3]Instituteof Ecological Conservation and Restoration,Chinese Academy of Forestry,Beijing 100091,China [4]Comprehensive Experimental Center of Chinese Academy of Forestry in Yellow River Delta,Dongying,Shandong 257000,China

出　　处：《Journal of Integrative Plant Biology》2025年第1期117-134,共18页植物学报(英文版)

基　　金：supported by the National Key Research and Development Program of China(Grant nos.2022YFD2201400 and 2016YFD0600105);the National Natural Science Foundation of China(Grant nos.32371826 and 32371841);the Key Research and Development Program of Shandong Province(Grant no.2023CXPT026)。

摘　　要：Poplar plantations are often established on nitrogen-poor land,and poplar growth and wood formation are constrained by low nitrogen(LN)availability.However,the molecular mechanisms by which specific genes regulate wood formation in acclimation to LN availability remain unclear.Here,we report a previously unrecognized module,basic region/leucine zipper 55(PtobZIP55)–PtoMYB170,which regulates the wood formation of Populus tomentosa in acclimation to LN availability.PtobZIP55 was highly expressed in poplar wood and induced by LN.Altered wood anatomical properties and increased lignification were detected in PtobZIP55-overexpressing poplars,whereas the opposite results were detected in PtobZIP55-knockout poplars.Molecular and transgenic analyses revealed that PtobZIP55 directly binds to the promoter sequence of PtoMYB170to activate its transcription.The phenotypes of PtoMYB170 transgenic poplars were similar to those of PtobZIP55 transgenic poplars under LN conditions.Further molecular analyses revealed that PtoMYB170 directly bound the promoter sequences of lignin biosynthetic genes to activate their transcription to increase lignin concentrations in LN-treated poplar wood.These results suggest that PtobZIP55 activates PtoMYB170 transcription,which in turn positively regulates lignin biosynthetic genes,increasing lignin deposition in the wood of P.tomentosa in the context of acclimation to LN availability.

关 键 词：lignin biosynthesis nitrogen deficiency POPULUS secondary xylem transcriptional regulation wood formation 

分 类 号：G63[文化科学—教育学]