不同地理种源甜龙竹土壤真菌群落结构与功能多样性  

Soil Fungal Community Structures and Functional Diversities of Different Geographical Provenances of Dendrocalamus brandisii

作　　者：陈倩 辉朝茂 陶航 覃万玲 王雨濛 肖正康 刘佳林 刘蔚漪 CHEN Qian;HUI Chaomao;TAO Hang;QIN Wanling;WANG Yumeng;XIAO Zhengkang;LIU Jialin;LIU Weiyi(College of Forestry,Southwest Forestry University,Academy of Bamboo and Rattan Sciences,Research Center of Clump Bamboo Engineering Technology,Kunming 650224,China)

机构地区：[1]西南林业大学林学院,竹藤科学研究院,丛生竹工程技术研究中心,昆明650224

出　　处：《土壤》2024年第6期1294-1303,共10页Soils

基　　金：云南省基础研究计划项目(202201AT070053);国家重点研发计划项目(2021YFD2200501);云南省农业联合专项项目(202301BD070001-123)资助。

摘　　要：为揭示甜龙竹(Dendrocalamus brandisii)不同地理种源土壤真菌群落的差异,以云南省保山昌宁(CN)、德宏芒市(MS)、临沧沧源(CY)、临翔马台(MT)、普洱思茅(SM)、红河石屏(SP)、玉溪新平(XP)7个地理种源地的甜龙竹土壤为研究对象,采用高通量测序和FUNGuild功能预测的研究方法对土壤真菌进行了测定与分析,探讨了主要土壤因子与真菌群落的相关性。结果表明:①不同地理种源甜龙竹土壤真菌群落α多样性和β多样性存在显著差异(P<0.05),其中沧源地区的真菌多样性显著高于其他6个地区,昌宁地区的真菌群落与其他6个地区的差异最大。②7个地理种源甜龙竹土壤真菌群落的优势菌群,在门水平上依次是子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和被孢菌门(Mortierellomycota),这些优势菌门占所有微生物的90%以上;在纲水平上依次是伞菌纲(Agaricomycetes)、粪壳菌纲(Sordariomycetes)、散囊菌纲(Eurotiomycetes)和银耳纲(Tremellomycetes);在属水平上,7个地区除石屏和新平以伞菌属(Agaricus)为优势属外,昌宁地区以unclassified_p_Ascomycota和古根菌属(Archaeorhizomyces),沧源地区以被孢霉属(Mortierella)和外瓶霉属(Exophiala),芒市地区以unclassified_c_Agaricomycetes和粗糙孔属(Trechispora),马台地区以青霉属(Penicillium)和unclassified_c_Sordariomycetes,思茅地区以Saitozyma和Apiotrichum,为优势属。③土壤pH(R^(2)=0.775,P=0.001)、有机质(R^(2)=0.627,P=0.001)、速效钾(R^(2)=0.548,P=0.002)和全氮(R^(2)=0.542,P=0.003)是影响土壤真菌群落的主要土壤因子。④通过FUNGuild功能比对发现,不同地理种源甜龙竹土壤真菌以腐生营养功能型为主,主要功能类群为真菌寄生-未定义的腐生菌。⑤昌宁地区在门、纲和属水平上均具有较多未鉴定的真菌,且有高达58.6%的真菌功能未能注释。综上,不同地理种源甜龙竹土壤真菌群落多样性、物种组成和真菌功能在空间尺度上存In order to reveal the differences in soil fungal communities of different geographical provenances of Dendrocalamus brandisii,soil samples under Dendrocalamus brandisii were collected from 7 different geographical locations in Yunnan Province,which were Changning County of Baoshan City(CN),Mangshi County of Dehong Prefecture(MS),Cangyuan County of Lincang City(CY),Matai Township of Linxiang District of Lincang City(MT),Simao District of Pu’er City(SM),Shiping County of Honghe Prefecture(SP),and Xinping County of Yuxi City(XP),the fungal community structures were analyzed by using high-throughput sequencing technology and FUNGuild functional prediction,and their correlation with main soil factors were explored.The results showed:1)There were significant differences inαdiversity andβdiversity of soil fungal community among different geographical provenances(P<0.05),in which CY was significantly higher than the other six regions,the fungal community structure of CN was most different from the other six regions;2)At the phylum level,Ascomycota,Basidiomycota and Mortierellomycota were dominant in soil fungal community of 7 geographical provenances,accounted for more than 90%of all microorganisms;At the class level,Agaricomycetes,Sordariomycetes,Eurotiomycetes and Tremellomycetes were dominant;At the genus level,Agaricus was the dominant in SP and XP,and the dominant genera were unclassified_p_Ascomycota and Archaeorhizomyces in CN,with Mortierella and Exophiala in CY,unclassified_c_Agaricomycetes and Trechispora in MS,Penicillium and unclassified_c_Sordariomycetes in MT,Saitozyma and Apiotrichum in SM;3)Soil pH(R^(2)=0.775,P=0.00),organic matter(R^(2)=0.627,P=0.001),available potassium(R^(2)=0.548,P=0.002)and total nitrogen(R^(2)=0.542,P=0.003)were the main factors affecting fungal community;4)Through FUNGuild functional comparison,it was found that soil fungal functional nutrition type of different geographical provenances of D.brandisii was mainly saprophytic nutrition type,and the main functional group was fun

关 键 词：甜龙竹 不同地理种源 真菌群落 功能预测 

分 类 号：S154[农业科学—土壤学]