一个小麦芒长基因的初步定位及候选基因预测  

Preliminary mapping of a wheat awn length gene and prediction of candidate genes

作　　者：杨晓雨 马指挥 魏青 牛志鹏 陈安琪 胡正冲 王林生[1,2] YANG Xiaoyu;MA Zhihui;WEI Qing;NIU Zhipeng;CHEN Anqi;HU Zhengchong;WANG Linsheng(College of Agriculture,Henan University of Science and Technology,Luoyang 471023,Henan,China;Luoyang Key Laboratory of Crop Genetic Improvement and Germplasm Innovation,Luoyang 471023,Henan,China)

机构地区：[1]河南科技大学农学院,河南洛阳471023 [2]洛阳市作物遗传改良与种质创新重点实验室,河南洛阳471023

出　　处：《浙江农业学报》2025年第1期14-23,共10页Acta Agriculturae Zhejiangensis

基　　金：河南省农业良种联合攻关项目(2022010104);河南省科技攻关项目(182102110207)。

摘　　要：小麦芒作为影响产量和抗逆性的重要农艺性状引起了许多小麦遗传改良工作者的广泛关注。本文以藁优2018(全芒)与科大102(顶芒)及其构建的F2分离群体为试验材料,在小麦抽穗期对其主要农艺性状进行统计分析,并利用SSR分子标记对小麦芒长基因进行初步定位。结果表明,小麦顶芒对全芒为显性单基因遗传,符合孟德尔分离规律。采用Joinmap4.0软件对芒长基因进行遗传连锁图谱的构建,最终目的基因被初步定位在5A染色体长臂末端的yzu443936和yzu454712两个标记之间。将这两个标记的引物序列与中国春V2.1基因组进行比对,得到两个标记间的物理距离为6.74 Mb(668.45~675.19 Mb)。通过IWGSC网站在该区间内共筛选到86个基因,其中有17个基因可能为调控芒长的候选基因。本研究结果可以为小麦芒长基因的精细定位及基因克隆提供理论基础。As an important agronomic trait affecting yield and stress resistance,wheat awn has attracted the attention of many wheat genetic improvement workers.In this paper,the main agronomic traits of Gaoyou 2018(whole awn),Keda 102(top awn)and their F2 isolated populations were statistically analyzed at the heading stage of wheat,and the wheat awn length gene was initially mapped using SSR molecular markers.The results showed that the top and whole awn of wheat were inherited by single dominant gene,which was in accordance with Mendelian separation rule.Joinmap4.0 software was used to construct the genetic linkage map of the awning gene,and the final target gene was initially located between the two markers yzu443936 and yzu454712 at the end of the long arm of chromosome 5A.The primers of these two markers were compared with the genome of Chinese spring V2.1,and the physical distance between the two markers was 6.74 Mb(668.45-675.19 Mb).A total of 86 genes were screened through the IWGSC website,among which 17 genes may be candidate genes for regulating awning length.This study provides a theoretical basis for fine localization and gene cloning of wheat awns.

关 键 词：普通小麦 芒长 遗传分析 候选基因 

分 类 号：S512[农业科学—作物学]