三种芍药属植物FLOWERING LOCUS T(FT)同源基因的克隆与表达分析  

Cloning and expression analysis of homologous FLOWERING LOCUS T(FT)genes from three species of Paeonia spp.

作　　者：李杏 刘燕[1] 高健洲[1] LI Xing;LIU Yan;GAO Jianzhou(College of Landscape Architecture,Beijing Forestry University,Beijing 100080,China)

机构地区：[1]北京林业大学园林学院,北京100080

出　　处：《浙江农业学报》2025年第1期90-102,共13页Acta Agriculturae Zhejiangensis

基　　金：雄安新区科技创新专项(2022XAGG0100)。

摘　　要：为探究FT基因在芍药属植物开花和种子下胚轴休眠解除中是否具有多效性,克隆得到川赤芍(Paeonia veitchii)、新疆芍药(P.anomala)和芍药凤凰涅槃(P.lactiflora‘Fenghuangniepan’)的FT同源基因(依次命名为PvFT、PaFT和PlFT1)的编码(coding sequence,CDS)序列,对它们进行生物信息学分析,并通过qRT-PCR技术分析3种芍药种子休眠解除和成花阶段FT基因的表达模式。结果表明,3种芍药属植物FT基因序列高度保守,CDS全长均为522 bp,编码173个氨基酸。系统进化分析显示,3个FT基因编码的蛋白质序列与牡丹的PsFT蛋白亲缘关系最近。时空特异性表达分析显示,3种芍药属植物种子中FT基因在种子下胚轴伸长时均上调表达,没有与下胚轴休眠期的长短表现出一致性;相应成株中PvFT和PlFT1基因分别在能开花的川赤芍的花期和凤凰涅槃的蕾期上调表达,而在不开花的川赤芍和新疆芍药中,PvFT和PaFT基因维持低水平表达。PaFT、PlFT1和PvFT基因在芍药属植物开花和种子下胚轴休眠解除中具有多效性,在成花和种子下胚轴伸长过程中起正调控作用。In order to explore whether the FT gene has pleiotropy in the flowering and seed hypocotyl dormancy release of Paeonia,the CDS(coding sequence)region of the FT homologous genes(named PvFT,PaFT,and PlFT1)of P.vetchii,P.anomala,and P.lactiflora‘Fenghuangniepan’were cloned and analyzed using bioinformatics,and the expression patterns of FT genes during the dormancy release and flowering stages of three peony germplasm seeds were analyzed using qRT-PCR technology.The results show that the FT gene sequences are highly conserved across the three Paeonia species,with a full CDS length of 522 bp encoding 173 amino acids.Phylogenetic analysis indicates that the proteins encoded by these three FT genes are most closely related to the PsFT protein from the peony.Temporal and spatial expression analysis reveal that in the seeds of three species of Paeonia,the expression levels of the FT genes upregulate during hypocotyl elongation,and there are no consistency with the length of the hypocotyl dormancy period.In corresponding adult plants,the expression levels of PvFT and PlFT1 genes are upregulated during the flowering stage of P.veitchii and the budding stage of P.lactiflora‘Fenghuangnipan’,while the expression levels of PvFT and PaFT genes are expressed at a low level during the non-flowering of P.veitchii and P.anomala.PaFT,PlFT1,and PvFT genes exhibit pleiotropic effects in the flowering and seed germination processes of Paeonia species,playing a positive regulatory role during flowering and seed hypocotyl elongation.

关 键 词：野生芍药 FT基因 克隆 开花 种子 下胚轴 

分 类 号：S682.12[农业科学—观赏园艺] Q786[农业科学—园艺学]