吡唑醚菌酯对铁皮石斛中多糖和黄酮的影响研究  

Effects of pyraclostrobin on polysaccharide and flavone in Dendrobium officinale

作　　者：黄浩[1,2] 汤涛 许振岚[2] 赵学平[2] HUANG Hao;TANG Tao;XU Zhenlan;ZHAO Xueping(College of Food and Health,Zhejiang A&F University,Hangzhou 311300,China;Key Laboratory for Pesticide Residue Detection,Ministry of Agriculture and Rural Affairs,Institute of Agri-Product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江农林大学食品与健康学院,浙江杭州311300 [2]浙江省农业科学院农产品质量安全与营养研究所,农业农村部农药残留检测重点实验室,浙江杭州310021

出　　处：《浙江农业学报》2025年第1期115-125,共11页Acta Agriculturae Zhejiangensis

基　　金：农产品标准化生产示范县建设(“一县一品一策”)重大专项(ZJZY2022001)。

摘　　要：本文研究了吡唑醚菌酯在铁皮石斛中的残留动态及其对多糖和黄酮含量的影响,并采用代谢组学探讨了吡唑醚菌酯对铁皮石斛多糖和黄酮含量影响的潜在机制。结果表明:末次施药后7 d,LC(推荐剂量处理)和HC(高剂量处理)组多糖含量相较BC(空白对照)组下降31%和38%(P<0.01),7~15 d回升至无显著差异直至采样结束;0~15 d,3组黄酮含量差异不显著,30~90 d,LC和HC组黄酮含量显著高于BC组,60 d差异最显著,分别升高31%和34%(P<0.01)。末次施药后7 d,LC和HC相较BC组多种代谢物含量显著上升,苯丙氨酸含量上升100%和70%(P<0.05),酪氨酸含量上升120%和97%(P<0.05),苯丙氨酸、酪氨酸和色氨酸合成,氮代谢等代谢通路显著上调;60 d,LC组相较BC组,根皮素含量、木犀草素含量、7-O-甲基圣草酚含量下调66%、18%和89%(P<0.05),黄酮和黄酮醇的生物合成与苯丙氨酸生物合成代谢通路显著下调,HC组与BC组间未出现差异富集代谢通路。结合残留动态推测,末次施药后7 d,LC和HC组铁皮石斛通过提升氨基酸浓度提高了铁皮石斛对吡唑醚菌酯胁迫的抗性,苯丙氨酸等黄酮前体物质含量的上升也促进了黄酮的合成;60 d LC组铁皮石斛应激反应逐渐减弱,HC组为应对较高浓度的吡唑醚菌酯残留,保持着较高的应激反应强度。7 d LC和HC组铁皮石斛可能促进多糖分解为可溶性糖以调节植株渗透压,从而降低了多糖含量。该研究结果可为铁皮石斛种植过程中的吡唑醚菌酯合理施用提供依据。In this paper,the residue dynamics of pyraclostrobin in Dendrobium officinale and its effect on the content of polysaccharide and flavone were studied,and the potential mechanism of the effect of pyraclostrobin on the content of polysaccharide and flavone in Dendrobium officinale was discussed by metabolomics.The results showed that on the 7th day after the last application,the polysaccharide content of LC(recommended dose treatment)and HC(high dose treatment)groups decreased by 31%and 38%(P<0.01)compared with BC(blank control)group,and increased to no significant difference on the 7th to 15th day until the end of sampling.There was no significant difference in the content of flavone among the three groups from 0 to 15 days,while the content of flavone in LC and HC groups was significantly higher than that in BC group from 30 to 90 days,and the difference was the most significant on the 60th day,with an increase of 31%and 34%,respectively(P<0.01).On the 7th day after the last application,the contents of several metabolites in LC and HC groups were significantly higher than those in BC group,phenylalanine increased by 100%and 70%(P<0.05),tyrosine increased by 120%and 97%(P<0.05),and metabolic pathways such as phenylalanine,tyrosine and tryptophan synthesis and nitrogen metabolism were significantly up-regulated;On the 60th day,the concentrations of phloretin,luteolin and 7-O-methyleriodictyol in LC group were 66%,18%and 89%lower than those in BC group(P<0.05),and the biosynthesis pathways of flavone and flavonols and phenylalanine were significantly down-regulated in LC group,while there was no differentially enriched pathway between HC group and BC group.According to the residue dynamics,the resistance of Dendrobium officinale to pyraclostrobin stress was improved by increasing the concentration of amino acids in LC and HC groups 7 days after the last application,and the increase of phenylalanine and other flavonoid precursors also promoted the synthesis of flavone.The stress response of Dendrobium officinale in

关 键 词：铁皮石斛 吡唑醚菌酯 多糖 黄酮 代谢 

分 类 号：S481.8[农业科学—农药学]