莴苣盘梗霉菌自然寄主专化性的分化及其对莴苣的侵染性  

Natural host-specific differentiation of Bremia lactucae and its infectivity to lettuce

作　　者：何晓婵 周小军 朱浩 龚婉 张敬泽[2] 朱丽燕 HE Xiaochan;ZHOU Xiaojun;ZHU Hao;GONG Wan;ZHANG Jingze;ZHU Liyan(Institute of Crop Cultivation and Plant Protection,Jinhua Academy of Agricultural Sciences,Jinhua 321017,Zhejiang,China;College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]金华市农业科学研究院作物栽培与植物保护研究所,浙江金华321017 [2]浙江大学农业与生物技术学院,浙江杭州310058

出　　处：《浙江农业学报》2025年第1期126-133,共8页Acta Agriculturae Zhejiangensis

基　　金：金华市科技计划(2021-2-002a)。

摘　　要：为明确引起浙江金华地区杂草苦苣菜和苣荬菜霜霉病的病原菌及其寄主范围,进行了病原菌鉴定和寄主范围的测定。依据形态学特征和分子特征鉴定、病原菌特异性引物PCR扩增结果,以及基于核糖体ITS区和28S基因、Cox2基因序列的系统发育分析,将引起2种杂草霜霉病的病原菌鉴定为莴苣盘梗霉菌。致病性试验结果显示,病原菌可侵染其他自然寄主和栽培品种莴苣和生菜,但不能侵染已知的自然寄主续断菊,证实来自杂草上的莴苣盘梗霉菌存在寄主专化性的分化,不完全相同于已知的寄主范围。苦苣菜和苣荬菜可能是莴苣霜霉病的接种源,控制这些杂草将是控制霜霉病害的重要一环,研究结果为揭示活体营养莴苣盘梗霉菌具有寄主专化性及其分化提供了直接证据。In order to reveal the host range of pathogen causing downy mildew of two weeds Sonchus oleraceus and S.wightianus in Jinghua City,Zhejiang Province,China,pathogen was identified and its host range was determined.According to morphological characteristics,analysis of PCR amplification results using pathogen-specific primers and phylogenetic analysis based on ribosome ITS region as well as 28S and Cox2 gene sequences,pathogen was identified as Bremia lactucae.Pathogenicity tests indicated that the pathogen was able to infect its other natural hosts and cultivated varieties such as Lactuca sativa var.angustana lrish ex Bremer and L.sativa var.ramose Hort.,but not infect a known host,Sonchus asper(L.)Hill,confirmed that existence of the host-specific differentiation in species B.lactucae,which was not identical to the known host range.This indicated that those two hosts might be important inoculum source for disease occurring in lettuce,highlighting the need for controlling these weeds to be an important part of controlling downy mildew,and it also provided direct evidence for the host specificity and differentiation of biotrophic B.lactucae.

关 键 词：莴苣 霜霉病 杂草 寄主专化性 莴苣盘梗霉菌 续断菊 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]