不同施氮处理下黑麦草翻压还田对滩涂盐渍土碳氮与细菌群落结构的影响  

Effects of overturning Lolium perenne under different nitrogen rates on carbon,nitrogen and bacterial community structure in saline soil of coastal area

作　　者：朱小梅[1] 邢锦城[1] 洪立洲[1] 王建红[2] 刘冲[1] 董静[1] 孙果丽 何苏南 ZHU Xiaomei;XING Jincheng;HONG Lizhou;WANG Jianhong;LIU Chong;DONG Jing;SUN Guoli;HE Sunan(Institute of Agriculture Sciences in the Coastal Area of Jiangsu,Yancheng 224002,Jiangsu,China;Institute of Soil,Fertilizer and Environmental Resources,Zhejiang Academy of Agriculture Sciences,Hangzhou 310021,China)

机构地区：[1]江苏沿海地区农业科学研究所,江苏盐城224002 [2]浙江省农业科学院环境资源与土壤肥料研究所,浙江杭州310021

出　　处：《浙江农业学报》2025年第1期159-168,共10页Acta Agriculturae Zhejiangensis

基　　金：国家现代农业产业技术体系资助项目(CARS-22-G-15);江苏省科技创新专项(BE2022304);江苏省现代农业产业技术体系项目(JAST〔2022〕245);江苏省沿海开发集团“揭榜挂帅”项目(2022YHTDJB014)。

摘　　要：为揭示不同施氮处理下黑麦草作绿肥翻压还田对滩涂盐渍土的改良效果,通过田间小区试验研究了0、90、150、210、270 kg·hm^(-2)施氮量(依次简记为CK、LN1、LN2、LN3、LN4)下黑麦草翻压还田对土壤碳氮和细菌群落结构的影响。结果表明:黑麦草生物量随施氮量的增加而增加,以LN4处理最高,为64227 kg·hm^(-2),植株碳、氮含量均以LN2处理较高,各处理的植株碳氮比无显著差异。黑麦草翻压还田后,与不施氮的对照相比,施氮处理的土壤pH值显著(P<0.05)降低,水溶性盐总量、有机碳含量、全氮含量、微生物生物量碳含量、微生物生物量氮含量显著提升。各处理相比,LN2的微生物熵最高,显著高于其他处理。在土壤微生物群落中,变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、拟杆菌门(Bacteroidetes)等菌群的相对丰度较高。各处理的土壤细菌香农(Shannon)指数和辛普森(Simpson)指数无显著差异,但Chao1指数和Ace指数均以LN2处理较高。总的来看,LN2处理下黑麦草翻压还田对降低滩涂盐渍土的pH值,提升土壤碳、氮含量,及改善土壤微生态环境的综合效应最佳。In order to explore the effect of overturning Lolium perenne as green manure on soil carbon,nitrogen and bacterial community structure under nitrogen application rates of 0,90,150,210,270 kg·hm^(-2)(based on pure N,denoted as CK,LN1,LN2,LN3,LN4,respectively),a field plot experiment was conducted in saline soil of coastal area.It was shown that the biomass of L.perenne increased with the elevated nitrogen application rates,and the highest biomass was found under the LN4 treatment,which was 64227 kg·hm^(-2).The carbon and nitrogen content of L.perenne was relatively higher under the LN2 treatment,and there was no significant difference in the C/N ratio among treatments.Compared with the control treatment without N application,the soil pH was significantly(P<0.05)decreased with nitrogen application,yet the contents of water-soluble salt,organic carbon,total nitrogen,microbial biomass carbon and microbial biomass nitrogen increased significantly.Among all the treatments,LN2 treatment exhibited the highest soil microbial quotient,which was significantly higher than the other treatments.In the soil,the relative abundance of Proteobacteria,Acidobacteria,Bacteroidetes was high.There was no significant difference in the Shannon index and Simpson index of soil bacteria among all the treatments,while the Chao1 index and Ace index of LN2 treatment was relatively higher.In general,LN2 treatment got the best comprehensive effect on reducing soil pH,increasing soil carbon and nitrogen content,and improving soil micro-ecological environment.

关 键 词：黑麦草 盐渍土 土壤细菌群落结构 

分 类 号：S147.2[农业科学—肥料学] S158.3[农业科学—农业基础科学]