PVDF层析膜的制备及在胶体金试纸的应用  

Preparation of PVDF chromatographic membrane and its application to colloidal gold test paper

作　　者：卞庆东 李永晖 汪朝晖 周玥 汪效祖 崔朝亮 BIAN Qingdong;LI Yonghui;WANG Zhaohui;ZHOU Yue;WANG Xiaozu;CUI Zhaoliang(State Key Laboratory of Materials-Oriented Chemical Engineering,College of Chemical Engineering,Nanjing Tech University,Nanjing 211800,China;National Engineering Research Center for Special Separation Membrane,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]南京工业大学化工学院,材料化学工程国家重点实验室,南京211800 [2]南京工业大学国家特种分离膜工程技术研究中心,南京211800

出　　处：《膜科学与技术》2025年第1期70-77,91,共9页Membrane Science and Technology

基　　金：国家重点研发计划项目(2023YFB3810502);国家自然科学基金项目(22078146)。

摘　　要：免疫层析技术常用硝酸纤维素(NC)膜,但其被国外垄断且易燃、稳定性差,而聚偏氟乙烯(PVDF)具有化学稳定性好、蛋白吸附强的优点.以PVDF为膜材料,聚乙烯吡咯烷酮(PVPK90)、聚乙二醇(PEG600)为添加剂,二甲基乙酰胺(DMAc)为溶剂,采用蒸汽诱导相转化法制膜.研究了添加剂PEG600用量、蒸汽诱导时间、蒸汽温度对膜结构、孔径、接触角及蛋白吸附量的影响;采用不同种类的表面活性剂对PVDF膜改性,通过动态接触角、毛细流动时间、蛋白吸附量对膜进行测试,并制备成试纸条.结果表明:当制膜条件为质量分数2%PEG600、蒸汽诱导时间20 min、蒸汽温度50℃时,膜的孔径约为7.4μm、接触角为85.1°、蛋白吸附量为22.6μg/cm^(2);用甲基椰油酰基牛磺酸钠(CMT)改性后,PVDF膜的毛细流动时间为199 s,蛋白吸附量达到75.7μg/cm^(2),且试纸条有显色能力.Nitrocellulose(NC)membrane is commonly used in immunochromatography,but it is monopolised by foreign countries and is flammable and unstable.Polyvinylidene fluoride(PVDF)has the advantages of good chemical stability and strong protein adsorption.PVDF as the membrane material,dimethylacetamide(DMAc)as the solvent,polyvinylpyrrolidone(PVPK90)and polyethylene glycol(PEG600)as additives,the membranes were prepared by the vapour-induced phase separation method.The influences of additive PEG600 content,vapour induction time and vapour temperature on membranes structure,pore size,contact angle,and protein adsorption were investigated;PVDF membranes were modified using different types of surfactants,and the membranes were tested by dynamic contact angle,capillary flow time,and protein adsorption which were then prepared as test strips.The results showed that when the membranes was made under the conditions of 2%PEG600,steam induction time of 20 min and steam temperature of 5o C,the pore size of the membranes was about 7.4μm,the contact angle was 85.1°,and the protein adsorption amount was 22.6μg/cm?,the capillary flow time of the PVDF membranes modified with sodium methylcocoyl taurate(CMT)was 199 s,and the protein adsorption amount reached 75.7μg/cm’,and the test strips had the ability to develop colour.

关 键 词：PVDF膜 蒸汽诱导相转化法 表面涂覆改性 侧流免疫层析 

分 类 号：TQ028.8[化学工程]