用于丝网印刷光电化学生物传感器的功能纳米材料的最新进展  

Recent Advances of Functional Nanomaterials for Screen-Printed Photoelectrochemical Biosensors

作　　者：杨美清 王路 卢浩滋 杨耀成[3] 刘松 Meiqing Yang;Lu Wang;Haozi Lu;Yaocheng Yang;Song Liu(Zoology Key Laboratory of Hunan Higher Education,College of Life and Environmental Science,Hunan University of Arts and Science,Changde 415000,Hunan Province,China;Institute of Chemical Biology and Nanomedicine(ICBN),State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Chemistry and Chemical Engineering,Hunan University,Changsha 410082,China;Department of Orthopaedics,The Second Xiangya Hospital,Central South University,Changsha 410011,China)

机构地区：[1]湖南文理学院生命与环境科学学院,湖南省动物学重点实验室,湖南常德415000 [2]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,化学生物学与纳米医学研究所,长沙410082 [3]中南大学湘雅二医院骨科,长沙410011

出　　处：《物理化学学报》2025年第2期32-47,共16页Acta Physico-Chimica Sinica

基　　金：湖南文理学院博士科研启动基金(21BSQD43);湖南省自然科学基金(2023JJ40463);国家自然科学基金(22175060)资助项目。

摘　　要：光电化学(PEC)生物传感器因其低背景、高灵敏度、高特异性和快响应速度等优点而备受关注。近年来,一次性丝网印刷电极(SPE)的引入极大地推动了PEC生物传感器的发展,使丝网印刷PEC生物传感器成为一种应用前景广阔的分析工具。在丝网印刷PEC生物传感器的构建过程中,光活性纳米材料起着至关重要的作用,因为它们不仅可用作光电转换平台,还可用作生物识别元件的装载平台。然而,单纯的光活性材料通常存在一些缺点,比如固有的毒性、宽带隙、高的电子空穴对重组率等,因此,通过各种设计策略来改善光活性材料的光电特性是十分必要的。为了获得高灵敏度的丝网印刷PEC生物传感器,通常还需要将高性能光电极与各种信号放大策略相结合。鉴于此,我们在本文中首次对用于丝网印刷PEC生物传感器的光活性材料进行了系统总结,并将其归为四大类:金属氧化物、金属硫族化合物、碳纳米材料和铋基纳米材料。同时,我们重点关注了光活性材料的设计策略,例如形态调控、元素掺杂、异质结构建等。此外,我们还通过具有代表性的丝网印刷PEC免疫传感器和丝网印刷PEC适体传感器介绍了一些信号放大策略,如酶标记放大(ELA)策略、聚合酶链反应(PCR)策略、滚环扩增(RCA)策略和杂交链式反应(HCR)策略。最后,我们还讨论了丝网印刷PEC生物传感器目前面临的挑战和前景。我们希望通过本文让读者全面了解丝网印刷PEC生物传感器的最新进展,并为该领域的未来发展提供可行性指导。Photoelectrochemical(PEC)biosensors have attracted intensive attention due to their advantages,including low background,high sensitivity,high specificity and rapid response.In recent years,the introduction of disposable screen-printed electrodes(SPE)has greatly facilitated the development of PEC biosensors,making screen-printed PEC biosensors a promising analytical tool for various applications.Photoactive nanomaterials play a crucial role in the construction of screen-printed PEC biosensors as they can be used not only as photoelectric conversion platforms but also as loading platforms for recognition elements.However,pure photoactive materials usually suffer from some drawbacks,such as inherent toxicity,wide bandgap,and high electron-hole pair recombination rate.Therefore,it is necessary to improve the photoelectric properties of these materials through various design strategies.Moreover,to obtain highly sensitive screen-printed PEC biosensors,it is usually necessary to combine the high-performance photoelectrodes with various signal amplification strategies.In view of this,we provide the first systematic summary of photoactive materials for screen-printed PEC biosensors in this paper,classifying them into four main categories:metal oxides,metal chalcogenides,carbon nanomaterials and bismuth-based nanomaterials.Meanwhile,we focus on the design strategies for photoactive materials,including morphology modulation,elemental doping,and heterostructure construction.In addition,we introduce signal amplification strategies,such as the enzyme label amplification(ELA)strategy,polymerase chain reaction(PCR)strategy,rolling circle amplification(RCA)strategy,and hybridization chain reaction(HCR)strategy,through representative screen-printed PEC immunosensors and screen-printed PEC aptasensors.Finally,we discuss the current challenges and prospects of screen-printed PEC biosensors.We hope to provide readers with a comprehensive understanding of the recent advances in screen-printed PEC biosensors and provide a feasible guid

关 键 词：光电化学分析 生物传感器 丝网印刷电极 光活性材料 信号放大技术 

分 类 号：O649[理学—物理化学]