催产素促进小鼠麻醉后觉醒和认知功能的恢复  

Oxytocin promotes arousal and cognitive functionrecovery in mice after anesthesia

作　　者：江文瑞 周禹涵 贾鸿熠 李依峣 周芳 马福娟 刘玉杰 张明明 JIANG Wenrui;ZHOU Yuhan;JIA Hongyi;LI Yiyao;ZHOU Fang;MA Fujuan;LIU Yujie;ZHANG Mingming(Department of Human Anatomy,Histology and Embryology,Basic Medical College,Air Force Medical University,Xi’an 710032;Department of Anesthesia,Xijing Hospital,Air Force Medical University,Xi’an 710032;91208 Military Hospital,Qingdao 266005,China)

机构地区：[1]空军军医大学基础医学院人体解剖与组织胚胎学教研室,西安710032 [2]空军军医大学西京医院麻醉科,西安710032 [3]中国人民解放军91208部队医院,青岛266005

出　　处：《神经解剖学杂志》2024年第6期695-702,共8页Chinese Journal of Neuroanatomy

基　　金：空军军医大学医学提升计划(2021HKYX06);西京医院“临床医学+X”研究中心科研课题(LHJJ24JH01)。

摘　　要：目的:通过观察小鼠在麻醉后翻正反射的消失(LORR)和恢复(RORR)情况,以及Y迷宫的探索情况,探究催产素(OXT)促进小鼠觉醒的作用。方法:采用免疫荧光染色的方法,验证蓝斑(LC)核团内酪氨酸羟化酶(TH)免疫阳性神经元的胞体和突起有催产素受体(OXTR)的表达。采用腹腔注射麻醉与气体麻醉的麻醉方式,以及腹腔注射给药、鞘内注射给药、右侧LC单管置管给药的给药方式,分别给予OXT或生理盐水作为实验组和对照组,探究小鼠给药后在诱导麻醉期和麻醉恢复期所需的时间,并对麻醉结果具有统计学差异的组别进行Y迷宫测试,探索RORR 6、9、18、24 h后Y迷宫新颖臂探索实验的探索情况,以及小鼠LC内TH的表达情况,并对上述结果进行统计分析。结果:LC内,有大量的TH与OXTR阳性神经元呈双重标记。腹腔注射和鞘内注射分别给予OXT和生理盐水的麻醉后RORR时间没有差异,单管套管给予OXT和生理盐水,腹腔麻醉和气体麻醉的LORR和RORR的时间也没有差异。与对照组相比,单管套管给予OXT的经气体麻醉的实验组小鼠麻醉恢复期所需时间减少(P<0.05)。该组小鼠麻醉恢复期结束9 h后Y迷宫新颖臂探索实验的探索活动增多(P<0.05),6、18、24 h的Y迷宫新颖臂探索实验的探索活动则没有明显的统计学差异。Western Blot结果显示,与对照组相比,实验组麻醉后9 h内LC中TH表达显著升高(P<0.05),9 h后则开始下降(P<0.01)。结论:OXT能够促进小鼠觉醒,且促进觉醒后9 h内的认知能力恢复。Objective:By observing the loss(LORR)and recovery of righting reflex(RORR)in mice after anesthesia and the exploration of Y-maze,explore the role of oxytocin(OXT)in promoting arousal in mice.Methods:The expression of oxytocin receptor(OXTR)in the cell bodies and tyrosine hydroxylase(TH)positive immunoreactive neurons in locus coeruleus(LC)was verified by immunofluorescence staining.Using the function of intraperitoneal injection anesthesia and gas anesthesia,and intraperitoneal injection,intrathecal injection,and right LC single tube catheter administration,OXT or saline given as experimental group and control group,respectively,explored the time needed in the induction anesthesia period and anesthesia recovery period of mice after administration,and conducted Y-maze test on groups with statistically different anesthesia results,then explored the exploration of novel arm in Y-maze after 6,9,18 and 24 hours of RORR.Results:In LC,TH and OXTR positive neurons had double labeling.There was no difference in the time of RORR after intraperitoneal injection and intrathecal injection of OXT and saline respectively,and no difference in the time of LORR and RORR after single-tube cannula administration of OXT and saline with intraperitoneal anesthesia and gas anesthesia.Compared with the control group,the recovery time of mice in the experimental group under gas anesthesia given OXT with single cannula was reduced(P<0.05).In this group,the exploration activities of Y-maze novel arm increased after 9 hours of anesthesia recovery(P<0.05),but there was no significant statistical difference in the exploration activities of Y-maze novel arm at 6,18 and 24 hours.Western Blot showed that compared with the control group,the expression of TH in LC in the experimental group increased significantly within 9 hours after anesthesia(P<0.05),and then began to decrease after 9 hours(P<0.01).Conclusion:OXT can promote the arousal of mice,and the cognitive degree of mice given OXT recovered better after waking up for 9 hours after anesthesi

关 键 词：蓝斑 催产素 麻醉 觉醒 小鼠 

分 类 号：R614[医药卫生—麻醉学]